{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:38:40Z","timestamp":1780706320354,"version":"3.54.1"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T00:00:00Z","timestamp":1581465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI18\/0089"],"award-info":[{"award-number":["PI18\/0089"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The causes of seronegative villous atrophy can be grouped as coeliac or noncoeliac related. There is no consensus on how to approach subjects with seronegative coeliac disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the accuracy of both an increase in CD3<jats:sup>+<\/jats:sup> T\u2010cell receptor gamma delta<jats:sup>+<\/jats:sup> (TCR\u03b3\u03b4<jats:sup>+<\/jats:sup>) intraepithelial lymphocytes and coeliac lymphogram for the diagnosis of coeliac disease in patients with seronegative villous atrophy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty\u2010seven consecutive patients with seronegative villous atrophy were included. Duodenal biopsies to assess TCR\u03b3\u03b4<jats:sup>+<\/jats:sup> and CD3<jats:sup>\u2212<\/jats:sup> by flow cytometry were performed at the index endoscopy. Coeliac lymphogram was defined as an increase in TCR\u03b3\u03b4<jats:sup>+<\/jats:sup> <jats:italic>plus<\/jats:italic> a decrease in CD3<jats:sup>\u2212<\/jats:sup> intraepithelial lymphocytes. Sensitivity, specificity and Fagan's nomogram were calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Coeliac disease was diagnosed in 37 patients and noncoeliac villous atrophy in 30. Coeliac patients were younger (39\u00a0\u00b1\u00a03 vs 55\u00a0\u00b1\u00a03\u00a0years; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.001), more often showed HLA\u2010DQ2\/8 (97.6% vs 61%; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.002) and had a more severe histology (61% vs 32% Marsh 3b\u2010c; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.055), as compared to noncoeliac ones. Coeliac lymphogram was associated with a sensitivity of 87% (CI, 73.7\u201095) and specificity of 96.7% (82.7\u201099.9), whereas evaluating only TCR\u03b3\u03b4<jats:sup>+<\/jats:sup> yielded a sensitivity of 91.3% (79.2\u201097.6) and specificity of 83.3% (65.3\u201094.3). Among patients with a pre\u2010test coeliac disease probability of 30%, post\u2010test probabilities were 92% and 5% for positive and negative coeliac lymphogram, and 70% and 4% for positive and negative TCR\u03b3\u03b4<jats:sup>+<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Coeliac lymphogram was associated with a high level of diagnostic evidence either against or in favour of coeliac disease in patients with seronegative villous atrophy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.15663","type":"journal-article","created":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T10:29:30Z","timestamp":1581503370000},"page":"699-705","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Gamma delta<sup>+<\/sup> intraepithelial lymphocytes and coeliac lymphogram in a diagnostic approach to coeliac disease in patients with seronegative villous atrophy"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1489-504X","authenticated-orcid":false,"given":"Fernando","family":"Fern\u00e1ndez\u2010Ba\u00f1ares","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Hospital Universitari Mutua Terrassa Terrassa Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de enfermedades hep\u00e1ticas y digestivas (CIBERehd) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Crespo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hospital Universitario Ram\u00f3n y Cajal Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9489-1603","authenticated-orcid":false,"given":"Concepci\u00f3n","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Laboratorio de investigaci\u00f3n en Gen\u00e9tica de enfermedades complejas Hospital Cl\u00ednico San Carlos IdISSC Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"L\u00f3pez\u2010Palacios","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hospital Cl\u00ednico San Carlos IdISSC Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Trist\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hospital Universitari Mutua Terrassa Terrassa Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de enfermedades hep\u00e1ticas y digestivas (CIBERehd) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Vivas","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Complejo Asistencial Universitario de Le\u00f3n Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Farrais","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hospital Fundaci\u00f3n Jim\u00e9nez D\u00edaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Arau","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hospital Universitari Mutua Terrassa Terrassa Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de enfermedades hep\u00e1ticas y digestivas (CIBERehd) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Vidal","sequence":"additional","affiliation":[{"name":"Department of flow cytometry. 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