{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T03:32:00Z","timestamp":1793417520493,"version":"4.5.0"},"reference-count":63,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T00:00:00Z","timestamp":1630540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005570","name":"Takeda Pharmaceuticals North America","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005570","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Irritable bowel syndrome (IBS) patients often experience meal\u2010associated symptoms. However, the underlying mechanisms are unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To determine small intestinal mechanisms of lipid\u2010induced symptoms and rectal hypersensitivity in IBS<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We recruited 26 IBS patients (12 IBS\u2010C, 14 IBS\u2010D) and 15 healthy volunteers (HV). <jats:italic>In vivo<\/jats:italic> permeability was assessed using saccharide excretion assay. Rectal sensitivity was assessed using a barostat before and after small bowel lipid infusion; symptoms were assessed throughout. Next, an extended upper endoscopy with probe\u2010based confocal laser endomicroscopy (pCLE) was performed with changes induced by lipids. Duodenal and jejunal mucosal biopsies were obtained for transcriptomics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Following lipid infusion, a higher proportion of HV than IBS patients reported no pain, no nausea, no fullness and no urgency (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05 for all). In a model adjusted for sex and anxiety, IBS\u2010C and IBS\u2010D patients had lower thresholds for first rectal sensation (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0007) and pain (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.004) than HV. <jats:italic>In vivo<\/jats:italic> small intestinal permeability and mean pCLE scores were similar between IBS patients and HV. Post\u2010lipid, pCLE scores were higher than pre\u2010lipid but were not different between groups. Baseline duodenal transient receptor potential vanilloid (TRPV) 1 and 3 expression was increased in IBS\u2010D, and TRPV3 in IBS\u2010C. Duodenal TRPV1 expression correlated with abdominal pain (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.51, <jats:italic>FDR<\/jats:italic>\u00a0=\u00a00.01), and inversely with first rectal sensation (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.48, <jats:italic>FDR<\/jats:italic>\u00a0=\u00a00.01) and pain (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.41, <jats:italic>FDR<\/jats:italic>\u00a0=\u00a00.02) thresholds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Lipid infusion elicits a greater symptom response in IBS patients than HV, which is associated with small intestinal expression of TRPV channels. TRPV\u2010mediated small intestinal chemosensitivity may mediate post\u2010meal symptoms in IBS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.16591","type":"journal-article","created":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T10:14:50Z","timestamp":1630577690000},"page":"1179-1192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Intestinal chemosensitivity in irritable bowel syndrome associates with small intestinal TRPV channel expression"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5092-0831","authenticated-orcid":false,"given":"Madhusudan","family":"Grover","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Berumen","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo 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