{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:36:10Z","timestamp":1790033770349,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Data on the role of the microbiome in adult patients with eosinophilic oesophagitis (EoE) are limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>To prospectively collect and characterise the salivary, oesophageal and gastric microbiome in patients with EoE, further correlating the findings with disease activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult patients with symptoms of oesophageal dysfunction undergoing upper endoscopy were consecutively enrolled. Patients were classified as EoE patients, in case of more than 15 eosinophils per high\u2010power field, or non\u2010EoE controls, in case of lack of eosinophilic infiltration. Before and during endoscopy, saliva, oesophageal and gastric fundus biopsies were collected. Microbiota assessment was performed by 16\u2009s rRNA analysis. A Sparse Partial Least Squares Discriminant Analysis (sPLS\u2010DA) was implemented to identify biomarkers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Saliva samples were collected from 29 EoE patients and 20 non\u2010EoE controls;, biopsies from 25 EoE and 5 non\u2010EoE controls. In saliva samples, 23 Amplicon Sequence Variants (ASVs) were positively associated with EoE and 27 ASVs with controls, making it possible to discriminate between <jats:styled-content style=\"fixed-case\">EoE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">non\u2010EoE<\/jats:styled-content> patients with a classification error (CE) of 24%. In a validation cohort, the accuracy, sensitivity, specificity, positive predictive value and negative predictive value of this model were 78.6%, 80%, 75%, 80% and 60%, respectively. Moreover, the analysis of oesophageal microbiota samples observed a clear microbial pattern able to discriminate between active and inactive EoE (CE\u00a0=\u00a08%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our preliminary data suggest that salivary metabarcoding analysis in combination with machine learning approaches could become a valid, cheap, non\u2010invasive test to segregate between EoE and non\u2010EoE patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.17091","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T05:35:13Z","timestamp":1655530513000},"page":"450-462","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Salivary microbiota composition may discriminate between patients with eosinophilic oesophagitis (<scp>EoE<\/scp>) and <scp>non\u2010EoE<\/scp> subjects"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6774-590X","authenticated-orcid":false,"given":"Sonia","family":"Facchin","sequence":"first","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology (DISCOG) University Hospital of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3056-518X","authenticated-orcid":false,"given":"Matteo","family":"Calgaro","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1786-1993","authenticated-orcid":false,"given":"Mattia","family":"Pandolfo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2798-552X","authenticated-orcid":false,"given":"Federico","family":"Caldart","sequence":"additional","affiliation":[{"name":"Department of Medicine, Gastroenterology Unit University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6026-5639","authenticated-orcid":false,"given":"Matteo","family":"Ghisa","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology (DISCOG) University Hospital of Padua  Padua Italy"},{"name":"Department of Oncological Gastrointestinal Surgery Gastroenterology Unit, S. Maria del Prato Hospital  Feltre Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4627-3445","authenticated-orcid":false,"given":"Eliana","family":"Greco","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology (DISCOG) University Hospital of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6785-9624","authenticated-orcid":false,"given":"Eleonora","family":"Sattin","sequence":"additional","affiliation":[{"name":"BMR Genomics srl  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4377-5685","authenticated-orcid":false,"given":"Giorgio","family":"Valle","sequence":"additional","affiliation":[{"name":"BMR Genomics srl  Padova 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