{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T23:05:03Z","timestamp":1756076703608,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T00:00:00Z","timestamp":1681948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>The diagnosis of alcoholic steatohepatitis (ASH) is based on liver biopsy, which is costly and invasive with non\u2010negligible morbidity. The aim of this study was to evaluate the accuracy of circulating cytokeratin 18 M65 fragment (K18\u2010M65) alone or in association with other markers for the non\u2010invasive diagnosis of ASH in patients ongoing alcohol withdrawal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study examined the serum level of K18\u2010M65 in a test cohort of 196 patients. All patients underwent liver biopsy, transient elastography (TE) and serum collection. The diagnostic accuracy of K18\u2010M65 alone or combined with clinico\u2010biological data was assessed and the best defined cut\u2010offs were validated in an independent validation cohort of 58 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>K18\u2010M65 had an area under the curve (AUC) of 0.82 (test cohort) and 0.90 (validation cohort). Using two cut\u2010off decision points, K18\u2010M65 was able to classify 46.9% (test cohort) and 34.5% (validation cohort) of patients with 95% sensitivity or specificity. Combining K18\u2010M65, alpha\u20102\u2010macroglobulin, TE, body mass index, and age, we created a score allowing accurate diagnosis of ASH with an AUC of 0.93 (test cohort) and 0.94 (validation cohort). This new score was able to rule out or rule in the diagnosis of steatohepatitis for probability \u22640.135 or \u22650.667 respectively in more than two\u2010thirds of patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We propose a new validated non\u2010invasive score for the diagnosis of ASH in patients ongoing alcohol withdrawal. This score can help to identify patients that may benefit from potential therapeutics or motivate them to reduce alcohol consumption.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.17515","type":"journal-article","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T14:37:49Z","timestamp":1682001469000},"page":"80-88","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Non\u2010invasive diagnosis of alcohol\u2010related steatohepatitis in patients ongoing alcohol withdrawal based on cytokeratin 18 and transient elastography"],"prefix":"10.1111","volume":"58","author":[{"given":"Arnaud","family":"Chalin","sequence":"first","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en sant\u00e9, environnement et travail) \u2010 UMR_S 1085  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Turlin","sequence":"additional","affiliation":[{"name":"Department of Pathology Universit\u00e9 Rennes 1, INRAE, INSERM, Institut NUMECAN (Nutrition Metabolisms and Cancer) \u2010 UMR_S 1241, CHU  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Ousmen","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health University Hospital  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Michalak","sequence":"additional","affiliation":[{"name":"Department of Pathology CHU  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Mueller","sequence":"additional","affiliation":[{"name":"Center for Alcohol Research University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5655-6778","authenticated-orcid":false,"given":"Sebastian","family":"Mueller","sequence":"additional","affiliation":[{"name":"Center for Alcohol Research University of Heidelberg  Heidelberg Germany"},{"name":"Bauchmedizin Viscera AG  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludivine","family":"Legros","sequence":"additional","affiliation":[{"name":"Service des Maladies du Foie CHU Rennes  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edouard","family":"Bardou\u2010Jacquet","sequence":"additional","affiliation":[{"name":"Service des Maladies du Foie CHU Rennes  Rennes France"},{"name":"Univ Rennes, INRAE, INSERM, CHU Rennes, Institut NUMECAN (Nutrition Metabolisms and Cancer)  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean Fran\u00e7ois","family":"Viel","sequence":"additional","affiliation":[{"name":"Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) \u2010 UMR_S 1085  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1673-2284","authenticated-orcid":false,"given":"Michel","family":"Samson","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en sant\u00e9, environnement et travail) \u2010 UMR_S 1085  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain","family":"Moirand","sequence":"additional","affiliation":[{"name":"Univ Rennes, INRAE, INSERM, CHU Rennes, Institut NUMECAN (Nutrition Metabolisms and Cancer)  Rennes France"},{"name":"UF Addictologie CHU Rennes  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,4,20]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Organization WH.Global status report on alcohol and health 2018.2019."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0014-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.03.018"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2009.02.003"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.12.022"},{"issue":"2","key":"e_1_2_10_7_1","first-page":"210","article-title":"Prognostic factors in alcoholic liver disease. 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