{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:13:12Z","timestamp":1787616792032,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Discriminating Crohn's disease (CD) with latent tuberculosis infection (LTBI) from intestinal tuberculosis (ITB) in tuberculosis\u2010endemic regions remains challenging.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To assess whether targeted next\u2010generation sequencing (tNGS) could be an efficient method for ITB diagnosis and discrimination from CD with LTBI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The study was conducted prospectively from September 2020 until December 2023. We recruited patients with undetermined intestinal ulcers and positive interferon\u2010gamma release assay. We compared tNGS (using fresh biopsy tissue samples from ulcer bases) to pathological detection of caseous necrotising granuloma, acid\u2010fast bacillus (AFB) staining, tuberculosis polymerase chain reaction (TB\u2010PCR) for diagnostic efficiency. ITB was diagnosed based on cure by anti\u2010tuberculosis therapy and comprehensive clinical evaluation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 100 patients included, 66 had ITB and 34 had CD with LTBI. The sensitivity, specificity, positive predictive value and negative predictive value of tNGS for ITB were 83% (72%\u201391%), 100% (87%\u2013100%), 100% (92%\u2013100%) and 76% (60%\u201387%), respectively. TNGS had significantly higher diagnostic sensitivity than AFB staining [15% (4%\u201339%),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05], TB\u2010PCR [22% (12%\u201336%),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05] and detection of caseous necrotising granulomas [17% (9%\u201328%),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05]. The models combining multiclinical factors increased sensitivity (97% vs. 83%) than tNGS alone. No patients with CD and LTBI had positive tNGS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>TNGS using fresh biopsy tissue from ulcer bases is highly sensitive and specific, with superiority over other traditional diagnostic methods for ITB detection. TNGS could facilitate rapid diagnosis of ITB and discrimination from CD with LTBI, particularly in high TB\u2010endemic countries.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apt.18522","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T02:33:52Z","timestamp":1738722832000},"page":"1218-1225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Crohn's Disease With Latent Tuberculosis Infection or Intestinal Tuberculosis: Rapid Discrimination by Targeted Next\u2010Generation Sequencing"],"prefix":"10.1111","volume":"61","author":[{"given":"Lingna","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Qiantang Branch of Sir Run Run Shaw Hospital College of Medicine Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushu","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Sir Run Run Shaw Hospital College of Medicine Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sir Run Run Shaw Hospital College of Medicine Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuwen","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Sir Run Run Shaw Hospital College of Medicine Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7938-7532","authenticated-orcid":false,"given":"Qian","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Sir Run Run Shaw Hospital College of Medicine Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-022-02171-7"},{"issue":"10","key":"e_1_2_8_3_1","first-page":"17578","article-title":"Differential Diagnosis Between Crohn's Disease and Intestinal Tuberculosis Using Integrated Parameters Including Clinical Manifestations, T\u2010SPOT, Endoscopy and CT Enterography","volume":"8","author":"Zhang T.","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12908"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v25.i4.418"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000025"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4597-8"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-1578(10)60515-6"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/1319-3767.199135"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd-20-1232"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.942073"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.04.048"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.802719"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0113-7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2995464"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy693"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2021.05.028"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/JS9.0000000000000500"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i20.2496"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14364"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00263-9"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105307"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01161-09"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.21.0305"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3233\/THC-240823"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000000064"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-58459-9"}],"container-title":["Alimentary Pharmacology &amp; 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