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The primary endpoint was HBsAg loss at Week 96. In Group II (pegylated interferon alpha\u20102b &amp; entecavir), 13 patients achieved HBsAg loss, whereas all patients in Group I (entecavir monotherapy) and 17 in Group II failed to achieve HBsAg loss.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At baseline, the level of clonal expansion was positively correlated with the duration of nucleos(t)ide analogue (NUC) treatment, the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, whereas no association was observed with the HBsAg, hepatitis B core\u2010related antigen (HBcrAg), HBV RNA or covalently closed circular DNA (cccDNA) levels. Patients with less than 28 breakpoint types achieved a significantly higher rate of HBsAg loss following Peg\u2010IFN\u2010based therapy at Week 48 and 96, compared to those with more than 28.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The duration of NUC treatment, as well as ALT and AST levels, were associated with the level of clonal expansion in virally\u2010suppressed patients. A lower number of breakpoint types at baseline may be related to a higher HBsAg loss rate following Peg\u2010IFN\u2010based treatment and serve as a useful marker in guiding treatment decision\u2010making (NCT02327416).<\/jats:p><\/jats:sec><jats:sec><jats:title>Trial Registration<\/jats:title><jats:p>The Anchor study is registered at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link>, NCT02327416<\/jats:p><\/jats:sec>","DOI":"10.1111\/apt.70270","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T03:30:02Z","timestamp":1752723002000},"page":"887-900","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["High\u2010Throughput Viral Integration Detection Reveals Baseline Breakpoints Burden Associated With <scp>HBsAg<\/scp> Seroclearance"],"prefix":"10.1111","volume":"62","author":[{"given":"Misi","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Disease, Tongji Hospital Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Disease, Tongji Hospital Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"You","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic 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