{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:24:00Z","timestamp":1792700640484,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32200428"],"award-info":[{"award-number":["32200428"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20220778"],"award-info":[{"award-number":["BK20220778"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Primary biliary cholangitis (PBC)\u2013related antibodies\u2014including anti\u2010mitochondrial antibodies (AMA), AMA\u2010M2, anti\u2010gp210, and anti\u2010sp100\u2014can occur in non\u2010PBC liver diseases. We evaluated single and combined antibody positivity, focusing on dual positivity, using liver biopsy as the reference standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective study included antibody\u2010positive patients who underwent liver biopsy. Patients were classified into three groups: only AMA(s) positive group, only anti\u2010gp210\/sp100 positive group, and dual\u2010antibody positive group. Clinical and biochemical characteristics were compared to evaluate diagnostic performance and disease severity. Antibody\u2010positive patients who did not initially meet diagnostic criteria for PBC were followed to assess diagnostic conversion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 733 antibody\u2010positive patients, 80.22% (588\/733) were diagnosed with PBC, while 19.78% (145\/733) were non\u2010PBC. Diagnostic rates were 80.05% (313\/391) in the only AMA(s) positive group, 53.85% (63\/117) in the only anti\u2010gp210\/sp100 positive group, and 94.22% (212\/225) in the dual antibody positive group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Dual antibody positive patients showed the most severe cholestatic profile, with significantly higher alkaline phosphatase, gamma\u2010glutamyl transferase, total bile acid, and immunoglobulin M levels. The only anti\u2010gp210\/sp100 positive group had the highest rate of gastrointestinal bleeding. Autoimmune hepatitis overlap was more common in the dual\u2010positive group than the only AMA(s) positive group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Among 145 non\u2010PBC patients followed for a median of 39.10\u2009months, 2.07% (3\/145) progressed to PBC, with a cumulative incidence of 7.40%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Histopathology remains the definitive standard for diagnosing PBC. Dual positivity for AMA\/AMA\u2010M2 and anti\u2010gp210\/sp100 showed the highest diagnostic value in identifying PBC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apt.70614","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:21:52Z","timestamp":1773807712000},"page":"1635-1645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Dual Positivity for\n                    <scp>AMA<\/scp>\n                    \/\n                    <scp>AMA<\/scp>\n                    \u2010\n                    <scp>M2<\/scp>\n                    and Anti\u2010gp210\/sp100 Shows Highest Diagnostic Value for Primary Biliary Cholangitis"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6633-6795","authenticated-orcid":false,"given":"Hong\u2010Li","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Teaching Hospital of Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Fan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao\u2010Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha\u2010Sha","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Clinical Teaching Hospital of Medical School, Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Teaching Hospital of Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Jun","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui\u2010Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Teaching Hospital of Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010Ling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Fang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; Outpatient, Follow\u2010Up Center, Nanjing Key Laboratory of Hepatology The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Dan","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Liver Disease &amp; 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