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Hepatocyte growth factor (HGF) was applied to primary human hepatocytes (PHH) and BA transporter expression was assessed by RT\u2010qPCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In two cohorts (Cohort 1: 164 AH, 63\u2009DC, 36 HC; Cohort 2: 94 AH, 175\u2009DC, 72 HC), total serum BAs were highest in AH (median concentration 186.0\u2009\u03bcM vs. 64.5\u2009DC vs. 5.0 HC), driven by elevated conjugated primary BAs (182.0\u2009\u03bcM vs. 54.0 vs. 2.2). Unconjugated primary BAs were highest in DC. Serum BAs distinguished AH from DC (Cohort 1 AUROC 0.964; Cohort 2 0.922;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Faecal BAs were reduced in AH (0.47\u2009mg\/g vs. 1.11\u2009DC vs. 2.64 HC); serum FGF19 elevated (5835\u2009pg\/mL AH vs. 865 jaundiced DC [bilirubin &gt;\u200980\u2009\u03bcmol\/L]). Serum conjugated BAs correlated negatively with NTCP expression (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200925, Spearman's rho \u22120.432,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.031). CYP7A1 was below the limit of detection. HGF was elevated in AH (7899\u2009pg\/mL vs. 2607\u2009DC,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). HGF treatment reduced PHH BSEP expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Serum conjugated primary BAs accumulate in AH. Elevated HGF may detrimentally affect the hepatoprotective adaptive reduction in NTCP\/increase in BSEP seen in cholestasis, contributing to the AH BA profile.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apt.70616","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:45:27Z","timestamp":1774280727000},"page":"1658-1672","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Disordered Bile Acid Metabolism in Alcohol\u2010Related Hepatitis"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2745-0282","authenticated-orcid":false,"given":"Luke D.","family":"Tyson","sequence":"first","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"},{"name":"Imperial College Healthcare NHS Trust, St Mary's Hospital, The Liver Unit  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Atkinson","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"},{"name":"Imperial College Healthcare NHS Trust, St Mary's Hospital, The Liver Unit  London UK"},{"name":"GSK  Brentford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6300-3100","authenticated-orcid":false,"given":"Benjamin H.","family":"Mullish","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"},{"name":"Imperial College Healthcare NHS Trust, St Mary's Hospital, The Liver Unit  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandros","family":"Pechlivanis","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Allison","sequence":"additional","affiliation":[{"name":"Cambridge NIHR Biomedical Research Centre, Addenbrooke's Hospital  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Austin","sequence":"additional","affiliation":[{"name":"Department of Hepatology Royal Derby Hospital  Derby UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Chappell","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart J.","family":"Forbes","sequence":"additional","affiliation":[{"name":"Centre for Regenerative Medicine, Institute for Regeneration and Repair The University of Edinburgh  Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewan H.","family":"Forrest","sequence":"additional","affiliation":[{"name":"Department of Hepatology Glasgow Royal Infirmary  Glasgow UK"},{"name":"University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alastair M.","family":"Kilpatrick","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"},{"name":"Centre for Regenerative Medicine, Institute for Regeneration and Repair The University of Edinburgh  Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Martinez\u2010Gili","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Masson","sequence":"additional","affiliation":[{"name":"Newcastle NIHR Biomedical Research Centre, Liver Unit, Newcastle upon Tyne Hospitals NHS Foundation Trust and Translational and Clinical Research Institute, Faculty of Medical Sciences Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5449-2424","authenticated-orcid":false,"given":"Mark J. 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