{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:43:34Z","timestamp":1747183414694,"version":"3.40.5"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T00:00:00Z","timestamp":1566259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003970","name":"Isfahan University of Medical Sciences","doi-asserted-by":"publisher","award":["395825"],"award-info":[{"award-number":["395825"]}],"id":[{"id":"10.13039\/501100003970","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Asian J Endoscop Surgery"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Some countries have implemented reuse of laparoscopic instruments for cost\u2010effective purposes. An accurate cleaning as the first step of reprocessing would lead to the effective sterilization. The purpose was to evaluate the effect of cleaning guidelines implementation on microbial load of laparoscopic instruments which were used in laparoscopic cholecystectomy surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/study.com\/academy\/lesson\/quasi-experimental-designs-definition-characteristics-types-examples.html\">experimental<\/jats:ext-link> study was done in an educational hospital, in 2017 and included a total of 128 laparoscopic instruments randomly selected from cholecystectomy surgeries and divided into two cleaning groups. The instruments were checked out in terms of number (colony\u2010forming units [CFU]\/mL) and type of microorganisms in two groups of routine cleaning and according to guideline cleaning. This guideline was indigenous and taken from successful instruction in this context that was presented by the Association for the Advancement of Medical Instrumentation (AAMI). The appropriate statistical analysis was conducted by SPSS version 19.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The average microbial load was 2.4\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> CFU\/100\u2009mL after clinical use. It was reduced to 7.2 \u00d7\u200910<jats:sup>5<\/jats:sup> CFU\/100\u2009mL in the control group and 3.4 \u00d7\u200910<jats:sup>4<\/jats:sup> CFU\/100\u2009mL in the intervention group, after the cleaning process. The most common microorganisms that were isolated immediately after clinical use were <jats:italic>Escherichia coli<\/jats:italic> 81.2%, <jats:italic>Pseudomonas<\/jats:italic> 68.8%, <jats:italic>Klebsiella<\/jats:italic> 57.8%, and spp., and so on.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The AAMI cleaning method is recommended to be utilized by operating room nurses for laparoscopic instruments.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ases.12750","type":"journal-article","created":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T13:21:06Z","timestamp":1566307266000},"page":"272-278","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of cleaning guidelines implementation on microbial colony count of laparoscopic instruments: A study in a public hospital in Iran"],"prefix":"10.1111","volume":"13","author":[{"given":"Akram","family":"Aarabi","sequence":"first","affiliation":[{"name":"Nursing and Midwifery Care Research Center, Operating Room Department, Faculty of Nursing and Midwifery Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9269-3673","authenticated-orcid":false,"given":"Sorour","family":"Mosleh","sequence":"additional","affiliation":[{"name":"Master Science in Perioperative Care, Operating Room Department Faculty of Nursing and Midwifery, Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Fazeli","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Farahmand","sequence":"additional","affiliation":[{"name":"Faculty Member of the Operating Room Department, Faculty of Nursing and Midwifery Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,20]]},"reference":[{"issue":"5","key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1097\/SLE.0b013e31817c7466","article-title":"Single\u2010use trocar: is it possible to reprocess it after the first use?","volume":"18","author":"Santos VSE","year":"2008","journal-title":"Surg Laparosc Endosc Percutan Tech"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005392-199909010-00038"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v3.i10.864"},{"volume-title":"Draft Guideline for Disinfection and Sterilization in Healthcare Facilities","year":"2002","author":"Rutala WA","key":"e_1_2_8_5_1"},{"volume-title":"Manual of Gynecological Laparoscopic Surgery","year":"2008","author":"Mencaglia L","key":"e_1_2_8_6_1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-11692011000200020"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1086\/590357"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-11692006000100010"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/408171"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ans.13856"},{"volume-title":"Comprehensive Guide to Steam Sterilization and Sterility Assurance in Health Care Facilities","year":"2006","author":"ANSI\/AAMI ST79: A, A2","key":"e_1_2_8_12_1"},{"volume-title":"WHO Guidelines for Safe Surgery 2009","year":"2009","author":"Lives SSS","key":"e_1_2_8_13_1"},{"key":"e_1_2_8_14_1","unstructured":"SwensonD. Laparoscopic instruments: Cleaning and testing methods [Online]. [cited 2013 May]; Available from:https:\/\/www.iahcsmm.org\/images\/Lesson_Plans\/CRCST\/CRCST130.pdf"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2016.02.004"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-69912013000300008"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/003335490712200205"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00464-009-0569-1"},{"issue":"12","key":"e_1_2_8_19_1","first-page":"586","article-title":"Carga microbiana de trocartes reprocess\u00e1veis ap\u00f3s laparoscopias ginecol\u00f3gicas","volume":"31","author":"Vilas\u2010Boas VA","year":"2009","journal-title":"Rev Bras Ginecol Obstet"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajme.2014.05.002"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2014.12.018"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2015.09.016"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2012.09.030"},{"key":"e_1_2_8_24_1","unstructured":"Isfahan University of Medical Sciences Infection Control Unit. Disinfection guideline [Online]. [cited 2017 Jul 15]; Available from:http:\/\/ta.mui.ac.ir\/taxonomy\/term\/68?page=4"},{"key":"e_1_2_8_25_1","unstructured":"Company Behbanshimi. Sayasept\u2010HI [Online]. [cited 2016 Nov 18] Available from:behbanshimi.ir\/sites\/medical\/en\/index.php\/products\/instruments-disinfectant\/item\/saya-sept-hi?category_id=7"},{"issue":"3","key":"e_1_2_8_26_1","first-page":"134","article-title":"Comparison of methods for the sterilization of instruments used for laparoscopic surgery","volume":"3","author":"Crema E","year":"2010","journal-title":"Bras J Video Sur"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2011.03.021"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2009.06.017"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2013.06.022"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aorn.2015.03.005"},{"key":"e_1_2_8_31_1","unstructured":"Association of Surgical Technologists (AST). Standards of Practice for the Decontamination of Surgical Instruments [Online]. [cited 2009 Apr 16]; Available from:http:\/\/www.ast.org\/uploadedFiles\/Main_Site\/Content\/About_Us\/Standard_Decontamination_%20Surgical_Instruments_.pdf"}],"container-title":["Asian Journal of Endoscopic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ases.12750","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ases.12750","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ases.12750","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T06:10:10Z","timestamp":1693980610000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ases.12750"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,20]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1111\/ases.12750"],"URL":"https:\/\/doi.org\/10.1111\/ases.12750","archive":["Portico"],"relation":{},"ISSN":["1758-5902","1758-5910"],"issn-type":[{"type":"print","value":"1758-5902"},{"type":"electronic","value":"1758-5910"}],"subject":[],"published":{"date-parts":[[2019,8,20]]},"assertion":[{"value":"2019-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}