{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T23:32:53Z","timestamp":1790465573631,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,11,19]],"date-time":"2012-11-19T00:00:00Z","timestamp":1353283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Porcine edema disease (<jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>) is caused by<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>higa toxin 2e\u2010producing<jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>scherichia coli<\/jats:italic>(<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>). Post\u2010weaned piglets often suffer from<jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>as a result of intestinal infection with<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>, which causes impaired growth performance and high mortality. Antimicrobial therapy is a curative treatment for piglets infected with<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>, but the emergence of antimicrobial\u2010resistant<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>has become a serious problem for Japanese pig farmers. Therefore, an alternative strategy other than antimicrobial therapy is needed for the prevention or treatment of<jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>. In this study, we evaluated the effect of oral administration of<jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>acillus subtilis<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">DB<\/jats:styled-content>9011 (<jats:styled-content style=\"fixed-case\">DB<\/jats:styled-content>9011) to prevent the experimental infection of<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>in weaning piglets. Eight 21\u2010day\u2010old piglets were divided into two groups:<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>challenge with the basal diet, and<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>challenge with<jats:styled-content style=\"fixed-case\">DB<\/jats:styled-content>9011 supplemented diet. The challenge was carried out when the animals were 25, 26 and 27 days old using<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>contained in capsules resistant against gastric digestion. All pigs were euthanized at 36 days of age.<jats:styled-content style=\"fixed-case\">DB<\/jats:styled-content>9011 improved the symptoms of<jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>and decreased the number of<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>in the ileal digesta and feces. Accordingly, oral administration of DB9011 in weaned piglets prevents<jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>through the suppression of the growth of<jats:styled-content style=\"fixed-case\">STEC<\/jats:styled-content>in the ileum.<\/jats:p>","DOI":"10.1111\/asj.12003","type":"journal-article","created":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T03:16:24Z","timestamp":1353381384000},"page":"316-321","source":"Crossref","is-referenced-by-count":21,"title":["The preventive effect of<i><scp>B<\/scp>acillus subtilus<\/i>strain<scp>DB<\/scp>9011 against experimental infection with enterotoxcemic<i><scp>E<\/scp>scherichia coli<\/i>in weaning piglets"],"prefix":"10.1111","volume":"84","author":[{"given":"Takamitsu","family":"Tsukahara","sequence":"first","affiliation":[{"name":"Kyoto Institute of Nutrition and Pathology Uji\u2010tawara Kyoto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Tsuruta","sequence":"additional","affiliation":[{"name":"Kyoto Institute of Nutrition and Pathology Uji\u2010tawara Kyoto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"KYODOKEN Institute Fushimi Kyoto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Hikita","sequence":"additional","affiliation":[{"name":"Agri\u2010Bio Business Department Idemitsu Kosan Co., Ltd Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masami","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Advanced Technology Research Laboratories Idemitsu Kosan Co., Ltd Sodegaura Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keizo","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Kyoto Institute of Nutrition and Pathology Uji\u2010tawara Kyoto"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.5.2344-2352.2002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.64.71"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2004.01478.x"},{"key":"e_1_2_6_5_1","first-page":"121","volume-title":"Progress in Pigs Science","author":"Fraser D","year":"1998"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5134-5146.2003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-006-9067-9"},{"key":"e_1_2_6_8_1","unstructured":"IizukaT UenoH.2007.Efficacy enhancer for alternative therapeutic agent and anticancer preparation making use of the same.International Patent PublicationNo. WO\/2007\/077606."},{"key":"e_1_2_6_9_1","first-page":"16","article-title":"Porcine edema disease","volume":"28","author":"Kobayashi H","year":"2006","journal-title":"All about SWINE"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00263-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-6226(94)90052-3"},{"key":"e_1_2_6_12_1","first-page":"3","article-title":"The use of Bacillus subtilis as an antidiarrhoeal microorganism","volume":"133","author":"Mazza P","year":"1994","journal-title":"Bollettino Chimico Farmaceutico"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.1093\/jn\/125.11.2838","article-title":"Diet\u2010dependent and diet\u2010independent metabolic responses underlie growth stasis of pigs at weaning","volume":"125","author":"McCracken BA","year":"1995","journal-title":"Journal of Nutrition"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/129.3.613"},{"key":"e_1_2_6_15_1","first-page":"142","article-title":"Effect of long\u2010term treatment with Bacillus subtilis on the frequency of urinary tract infections in older patients","volume":"2","author":"Meroni PL","year":"1983","journal-title":"Chemioterapia"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.11.3156-3161.2001"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.69.103"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.49.414"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.67.1167"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2003.tb02786.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.66.899"},{"key":"e_1_2_6_22_1","first-page":"70715","article-title":"Consideration of the human health impact of the microbial effects of antimicrobial new animal drugs intended for use in food producing animals. 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