{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T12:05:14Z","timestamp":1773662714541,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,3,24]],"date-time":"2013-03-24T00:00:00Z","timestamp":1364083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study investigated the effects of skim milk on the quality and fertility of boar spermatozoa under long\u2010term chilled preservation. Semen samples were stored in <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>odena solution supplemented with 0 (control) to 50\u2009<jats:styled-content style=\"fixed-case\">mg\/mL<\/jats:styled-content> skim milk at 5\u00b0<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> for 4 weeks; spermatozoa stored with 7.5 and 15\u2009<jats:styled-content style=\"fixed-case\">mg\/mL<\/jats:styled-content> of skim milk (7.5\u2010<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> and 15\u2010<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> groups, respectively) exhibited significantly higher motility indices than those of the control group up to 3 weeks (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009&lt;\u20090.05), and the 7.5\u2010<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> group showed improved motility indices even after 4 weeks (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009&lt;\u20090.05). <jats:italic>In vitro<\/jats:italic> fertilization using spermatozoa in the 7.5\u2010<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> and 15\u2010<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> groups stored at 5\u00b0<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> for 2 weeks showed significantly higher fertilization rates of spermatozoa and the development rates to blastocyst than the control group (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009&lt;\u20090.05), and the 7.5\u2010<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> group showed similar rates of fertilization and blastocyst formation in the fresh non\u2010stored spermatozoa group. After artificial insemination using spermatozoa stored for 2 weeks in the 7.5\u2010<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> group, healthy piglets were obtained. Boar spermatozoa can be stored at 5\u00b0<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> in a <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>odena solution containing skim milk. Supplementation of 7.5\u2009<jats:styled-content style=\"fixed-case\">mg\/mL<\/jats:styled-content> skim milk improves boar spermatozoa motility and fertility even after liquid preservation at 5\u00b0<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> for 2 weeks.<\/jats:p>","DOI":"10.1111\/asj.12049","type":"journal-article","created":{"date-parts":[[2013,3,25]],"date-time":"2013-03-25T03:13:40Z","timestamp":1364181220000},"page":"600-606","source":"Crossref","is-referenced-by-count":10,"title":["Motility and fertility of boar semen after liquid preservation at 5\u00b0<scp>C<\/scp> for more than 2 weeks"],"prefix":"10.1111","volume":"84","author":[{"given":"Zhao","family":"Namula","sequence":"first","affiliation":[{"name":"The United Graduate School of Veterinary Science Yamaguchi University  Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Sato","sequence":"additional","affiliation":[{"name":"The United Graduate School of 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