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A total of 28\u2009803\u2009759 bp of raw sequence data was generated by 454 <jats:styled-content style=\"fixed-case\">GS F<\/jats:styled-content>lx+. After removal of adaptor sequences, 28\u2009730\u2009361 bp remained and 117\u2009279 reads were obtained, with an average length of 244 bases. Simultaneously, complementary <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples from two different reproductive stages of goose ovarian, hypothalamus and pituitary tissue were sequenced separately using <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>llumina <jats:styled-content style=\"fixed-case\">MiSeq<\/jats:styled-content> platform. A total of 12\u2009688\u2009673\u2009148 bp of raw sequence data were generated by Illumina <jats:styled-content style=\"fixed-case\">MiSeq<\/jats:styled-content>. After removal of adaptor sequences, 8\u2009198\u2009126\u2009562 bp remained and 60\u2009382\u2009786 clean reads were obtained, with an average length of 135 bases. Assembly of all the reads from both 454 <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>lx+ and <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>llumina platforms formed 56\u2009839 contigs. The sequence size ranges from 38 to 28\u2009206 bp in size, with an average size of 2584 bp and an <jats:styled-content style=\"fixed-case\">N50<\/jats:styled-content> of 4624. The assembly produced a substantial number of large contigs: 35\u2009545 (62.5%) were longer than 1\u2009kb, of which 8850 (15.6%) were longer than 5\u2009kb. The sequencing depth was 85 <jats:styled-content style=\"fixed-case\">X<\/jats:styled-content> on average. We performed comprehensive function annotations on unigenes including protein sequence similarity, gene ontology (<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content>) term classification, and Kyoto Encylcopedia of Genes and Genomes (<jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content>) pathway enrichment. <jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content> analysis showed that approximately 63% of the contigs had annotation information, among the 35\u2009953 annotated isotigs in <jats:styled-content style=\"fixed-case\">Nr<\/jats:styled-content> database, 24\u2009783 (68.9%) sequences were assigned with one or more <jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content> terms. There were 14\u2009634 (40.7%) isotigs for biological processes, 10\u2009557(29.3%) isotigs for cellular component, 22\u2009607 (62.9%) isotigs for molecular function. The result of <jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content> pathway mapping 8926 sequences had the pathway annotation, and took part in 477 pathways. Additionally, 10\u2009685 simple sequence repeat (<jats:styled-content style=\"fixed-case\">SSR<\/jats:styled-content>) markers were identified from the assembled sequences. The most frequent repeat motifs were trinucleotides, which accounted for 53.03% of all <jats:styled-content style=\"fixed-case\">SSR<\/jats:styled-content>s, followed by dinucleotides (39.9%), tetranucleotides (5.08%), pentanucleotides (1.68%) and hexanucleotides (0.32%). Transcriptome sequencing on mixture issue of the geese yielded substantial transcriptional sequences and potentially useful <jats:styled-content style=\"fixed-case\">SSR<\/jats:styled-content> markers which provide an important data source for geese research.<\/jats:p>","DOI":"10.1111\/asj.12197","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T03:48:35Z","timestamp":1397447315000},"page":"650-659","source":"Crossref","is-referenced-by-count":17,"title":["Comprehensive analysis of <scp>S<\/scp>ichuan white geese (<i><scp>A<\/scp>nser cygnoides<\/i>) transcriptome"],"prefix":"10.1111","volume":"85","author":[{"given":"Ning","family":"Ding","sequence":"first","affiliation":[{"name":"Chongqing Academy of Animal Science  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Han","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science  Chongqing 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