{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T13:48:29Z","timestamp":1762955309911},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,7,14]],"date-time":"2014-07-14T00:00:00Z","timestamp":1405296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pigs throughout the world are afflicted with leptospirosis, causing serious economic losses and potential hazards to human health. Although it has been known that leptospiral lipopolysaccharide (<jats:styled-content style=\"fixed-case\">L\u2010LPS<\/jats:styled-content>) is involved in an immunological reaction between an antigen and a host cell, little is known about how the immune system of pigs can respond to <jats:styled-content style=\"fixed-case\">L\u2010LPS<\/jats:styled-content>. Here, we stimulated pig fibroblasts by <jats:styled-content style=\"fixed-case\">L\u2010LPS<\/jats:styled-content> and then quantitatively measured gene and protein expression levels of two toll\u2010like receptors (<jats:styled-content style=\"fixed-case\">TLRs<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">TLR2<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TLR4<\/jats:styled-content>, by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blotting. As a result, expression of <jats:styled-content style=\"fixed-case\">TLR2<\/jats:styled-content> was found to be significantly up\u2010regulated within 24\u2009h after <jats:styled-content style=\"fixed-case\">L\u2010LPS<\/jats:styled-content> stimulation whereas induction of TLR4 expression was relatively weak. We also revealed that of myeloid differentiation primary response gene 88 (<jats:styled-content style=\"fixed-case\">MyD88<\/jats:styled-content>), interleukin 6 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106) and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 gene expressions were markedly up\u2010regulated by <jats:styled-content style=\"fixed-case\">L\u2010LPS<\/jats:styled-content> stimulation. These results may suggest that the pig cell can activate <jats:styled-content style=\"fixed-case\">TLR2<\/jats:styled-content> rather than <jats:styled-content style=\"fixed-case\">TLR4<\/jats:styled-content> by <jats:styled-content style=\"fixed-case\">L\u2010LPS<\/jats:styled-content> stimulation, thereby inducing expression of cytokines.<\/jats:p>","DOI":"10.1111\/asj.12254","type":"journal-article","created":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T17:12:57Z","timestamp":1405444377000},"page":"238-244","source":"Crossref","is-referenced-by-count":10,"title":["Leptospiral lipopolysaccharide stimulates the expression of toll\u2010like receptor 2 and cytokines in pig fibroblasts"],"prefix":"10.1111","volume":"86","author":[{"given":"Yijie","family":"Guo","sequence":"first","affiliation":[{"name":"Laboratory of Animal Microbiology Graduate School of Agricultural Science Tohoku University  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomokazu","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Breeding and Genetics Graduate School of Agricultural Science Tohoku University  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichiro","family":"Donai","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Breeding and Genetics Graduate School of Agricultural Science Tohoku University  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kengo","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Microbiology Graduate School of Agricultural Science Tohoku University  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mizuki","family":"Masuda","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Microbiology Graduate School of Agricultural Science Tohoku University  Sendai 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Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.1310"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6799-6807.2003"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/m92-047"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.02.017"},{"key":"e_1_2_5_7_1","volume-title":"Leptospira and Leptospirosis","author":"Faine 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