{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:57:38Z","timestamp":1788872258676,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,11,20]],"date-time":"2014-11-20T00:00:00Z","timestamp":1416441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2010\/10502-5"],"award-info":[{"award-number":["2010\/10502-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Biofilm formation and antimicrobial resistance of\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\n                      taphylococcus aureus\n                    <\/jats:italic>\n                    are important virulence factors in cases of mastitis in dairy cows. However, few studies have investigated mastitis strains isolated from heifers. Within this context, the objective of the present study was to investigate biofilm formation on\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    ongo red agar, the presence of the\n                    <jats:italic>\n                      ica\n                      <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      ica\n                      <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                    <\/jats:italic>\n                    genes by polymerase chain reaction (\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    ), and the percentage of\n                    <jats:italic>in vitro<\/jats:italic>\n                    antimicrobial resistance of 110\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\n                      .\u2009aureus\n                    <\/jats:italic>\n                    isolates from mammary gland secretions of heifers and cows with mastitis.\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    detected the\n                    <jats:italic>\n                      ica\n                      <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    <\/jats:italic>\n                    and i\n                    <jats:italic>\n                      ca\n                      <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                    <\/jats:italic>\n                    genes in 98% and 100% of isolates, respectively. However, only 55.5% of all isolates produced a biofilm on\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    ongo red agar. Antimicrobial susceptibility testing revealed that 47.0% of isolates from heifers and 70.4% of isolates from cows were resistant to at least one of the antimicrobial agents tested. Resistance to penicillin and\/or ampicillin was the most frequent (44.5%). These results indicate the need to implement prophylactic and control measures of mastitis for heifers. Heifers and cows can carry resistant strains with the capacity of biofilm production, a fact representing a threat to public health and animal well\u2010being and generating losses to dairy farmers.\n                  <\/jats:p>","DOI":"10.1111\/asj.12284","type":"journal-article","created":{"date-parts":[[2014,11,20]],"date-time":"2014-11-20T05:55:25Z","timestamp":1416462925000},"page":"340-344","source":"Crossref","is-referenced-by-count":17,"title":["Investigation of biofilm production and\n                    <i>\n                      ica\n                      <scp>A<\/scp>\n                    <\/i>\n                    and\n                    <i>\n                      ica\n                      <scp>D<\/scp>\n                    <\/i>\n                    genes in\n                    <i>\n                      <scp>S<\/scp>\n                      taphylococcus aureus\n                    <\/i>\n                    isolated from heifers and cows with mastitis"],"prefix":"10.1111","volume":"86","author":[{"given":"L\u00edvia","family":"Castelani","sequence":"first","affiliation":[{"name":"ICB\/USP \u2013 Institute of Biomedical Sciences Department of Microbiology University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"IZ \u2013 Instituto de Zootecnia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Eduardo","family":"Pilon","sequence":"additional","affiliation":[{"name":"IZ \u2013 Instituto de Zootecnia  S\u00e3o Paulo Brazil"},{"name":"UNESP \u2013 Universidade Estadual Paulista Julio de Mesquita Filho  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thamires","family":"Martins","sequence":"additional","affiliation":[{"name":"IZ \u2013 Instituto de Zootecnia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Rodrigues","family":"Pozzi","sequence":"additional","affiliation":[{"name":"IZ \u2013 Instituto de Zootecnia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Rodrigues Pozzi","family":"Arcaro","sequence":"additional","affiliation":[{"name":"IZ \u2013 Instituto de Zootecnia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(01)00383-2"},{"key":"e_1_2_6_3_1","volume-title":"Compendium of Methods for the Microbiological Examination of Foods","author":"APHA","year":"2001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2151-2156.2001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"4857","DOI":"10.1128\/iai.61.11.4857-4862.1993","article-title":"Phase variation of slime production in Staphylococcus aureus: implications in colonization and virulence","volume":"61","author":"Baselga R","year":"1993","journal-title":"Infection and Immunity"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822009000200009"},{"key":"e_1_2_6_7_1","unstructured":"CLSI (Clinical and Laboratory Standards).2012.Performance standards for antimicrobial susceptibility testing; twenty\u2010second informational supplement32(3)."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2009000500002"},{"key":"e_1_2_6_9_1","unstructured":"ConaginA NagaiV Ambr\u00f3sioLA.2008.Princ\u00edpios de t\u00e9cnica experimental e an\u00e1lise estat\u00edstica de experimentos. 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