{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:42:18Z","timestamp":1774438938635,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T00:00:00Z","timestamp":1417651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2015,5]]},"abstract":"Abstract<\/jats:title>B<\/jats:styled-content>an is an endangered miniature pig breed in V<\/jats:styled-content>ietnam. This study aimed to set up an in vitro<\/jats:italic> embryo production (IVP<\/jats:styled-content>) system for this breed. B<\/jats:styled-content>an's epididymal sperm concentration (1240\u2009\u00b1\u200935\u2009\u00d7\u2009106<\/jats:sup>\/mL) was lower (P<\/jats:italic>\u2009<\u20090.01) compared with Landrace (4160\u2009\u00b1\u200942\u2009\u00d7\u2009106<\/jats:sup>\/mL). However, sperm characteristics before and after freezing in B<\/jats:styled-content>an and L<\/jats:styled-content>andrace were similar. The numbers of follicles with diameter larger than 2\u2009mm per ovary in B<\/jats:styled-content>an females treated with equine chorionic gonadotropin and human chorionic gonadotropin (27.1\u2009\u00b1\u20091.3) were higher (P<\/jats:italic>\u2009<\u20090.05) than those in L<\/jats:styled-content>andrace (12.9\u2009\u00b1\u20092.0) and in non\u2010hormone stimulated B<\/jats:styled-content>an (no >\u20092\u2009mm follicles). After in vitro<\/jats:italic> maturation, the percentages of oocytes with expanded cumulus cells and the first polar body (matured oocytes) were not different among B<\/jats:styled-content>an, hormone\u2010stimulated B<\/jats:styled-content>an and L<\/jats:styled-content>andrace. The percentages of two\u2010cell embryos and morulae derived from oocytes collected from three sources did not differ. However, the rate of blastocysts derived from oocytes in non\u2010stimulated B<\/jats:styled-content>an (4.0\u2009\u00b1\u20093.8%) was lower (P<\/jats:italic>\u2009<\u20090.05) than that in Landrace (15.3\u2009\u00b1\u20091.8%). In conclusion, an effective IVP<\/jats:styled-content> system for good quality embryos in B<\/jats:styled-content>an, that is essential for genetic conservation of this breed, was established.<\/jats:p>","DOI":"10.1111\/asj.12317","type":"journal-article","created":{"date-parts":[[2014,12,6]],"date-time":"2014-12-06T00:27:03Z","timestamp":1417825623000},"page":"487-493","source":"Crossref","is-referenced-by-count":8,"title":["Production of B<\/scp>an miniature pig embryos by in vitro<\/i> fertilization: A comparative study with L<\/scp>andrace"],"prefix":"10.1111","volume":"86","author":[{"given":"Bui Xuan","family":"Nguyen","sequence":"first","affiliation":[{"name":"Laboratory of Embryo Biotechnology and Stem Cells Institute of Biotechnology Vietnamese Academy of Science and Technology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences National Institute of Agrobiological Sciences Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi","family":"Uoc","sequence":"additional","affiliation":[{"name":"Laboratory of Embryo Biotechnology and Stem Cells Institute of Biotechnology Vietnamese Academy of Science and Technology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thanh Quang","family":"Dang\u2010Nguyen","sequence":"additional","affiliation":[{"name":"Laboratory of Embryo Biotechnology and Stem Cells Institute of Biotechnology Vietnamese Academy of Science and Technology Hanoi Vietnam"},{"name":"Veterinary Research Institute Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Viet","family":"Linh","sequence":"additional","affiliation":[{"name":"Laboratory of Embryo Biotechnology and Stem Cells Institute of Biotechnology Vietnamese Academy of Science and Technology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi","family":"Men","sequence":"additional","affiliation":[{"name":"Laboratory of Embryo Biotechnology and Stem Cells Institute of Biotechnology Vietnamese Academy of Science and Technology Hanoi Vietnam"},{"name":"Division of Animal Sciences National Institute of Agrobiological Sciences Tsukuba Japan"},{"name":"Graduate School of Life and Environmental Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trung Thanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Laboratory of Embryo Biotechnology and Stem Cells Institute of Biotechnology Vietnamese Academy of Science and Technology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Nagai","sequence":"additional","affiliation":[{"name":"Food and Fertilizer Technology Center Taipei Taiwan"},{"name":"Department of Agricultural Biotechnology College of Agriculture and Life Sciences Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00201-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD07018"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.09-165K"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.1.49"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod54.6.1412"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00166-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.4.1033"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00655-6"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(89)90089-7"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01190.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12097"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0840585"},{"key":"e_1_2_6_14_1","first-page":"19","volume-title":"Manual for Cryopreservation of Pig Spermatozoa","author":"Niwa T","year":"1989"},{"key":"e_1_2_6_15_1","first-page":"61","article-title":"Culture of pig embryos","volume":"48","author":"Petters RM","year":"1993","journal-title":"Journal of Reproduction and Fertility. 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