{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T17:11:37Z","timestamp":1779297097774,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,11,30]],"date-time":"2014-11-30T00:00:00Z","timestamp":1417305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The high incidence of polyspermy is one of the major obstacles during <jats:italic>in vitro<\/jats:italic> fertilization (<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content>) in pigs. To overcome this, we developed a novel <jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content> method, which involves constant rotation. Oocytes matured <jats:italic>in vitro<\/jats:italic> were mixed with spermatozoa (0.2\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> sperm\/mL) in an <jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content> medium (200\u2009\u03bcL) using a 200\u2009\u03bcL <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> tube. This tube was then rotated at 1\u2009rpm for 6\u2009h at 38.5\u00b0<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> in a rotation mixer (experimental group). A second <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> tube was simultaneously cultured without rotation (control group). The rate of polyspermy was evaluated 12\u2009h after insemination and was significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05; 21.0% vs. 48.3%) lower in the experimental group than in the control group. Sperm penetration rate was similar in oocytes from the experimental and control groups (75.2% vs. 83.1%). However, monospermic fertilization rate of the oocytes was significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05; 44.8% vs. 21.2%) higher in the experimental group than in the control group. Furthermore, the rate of blastocyst formation (30.1% vs. 20.8%) increased in the experimental group, as compared to the control group. This present system will contribute to increase the efficacy of blastocyst production through reduction of polyspermic penetration.<\/jats:p>","DOI":"10.1111\/asj.12327","type":"journal-article","created":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T03:01:23Z","timestamp":1417402883000},"page":"494-498","source":"Crossref","is-referenced-by-count":15,"title":["A new rolling culture\u2010based <i>in vitro<\/i> fertilization system capable of reducing polyspermy in porcine oocytes"],"prefix":"10.1111","volume":"86","author":[{"given":"Hideki","family":"Kitaji","sequence":"first","affiliation":[{"name":"Laboratory of Animal Reproduction Faculty of Agriculture Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoji","family":"Ookutsu","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction Faculty of Agriculture Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Sato","sequence":"additional","affiliation":[{"name":"Section of Gene Expression Regulation Frontier Science Research Center Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuchika","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction Faculty of Agriculture Kagoshima University  Kagoshima 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