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In typical systemic anaphylaxis models, animals are challenged with large quantity of antigens via an intravenous (i.v.) route. Here, we showed a simple anaphylactic shock after challenge via intraperitoneal (i.p.) route. The i.p. method is simpler than i.v. administration and has a lower risk for failure. To generate this anaphylactic model, 5\u2010week\u2010old female BALB\/c mice were first i.p. sensitized with buckwheat antigen mixed with B\u2010type CpG\u2010ODN. After 2 weeks, mice were challenged with antigen to induce anaphylactic shock, which was evaluated by scoring the severity symptoms and measuring serum levels of various proteins and splenic cell producing cytokines. Immunoglobulin (Ig)G<jats:sub>2a<\/jats:sub> production and interferon\u2010\u03b3 positive cells were markedly increased in mice immunized with antigen mixed with B\u2010type CpG\u2010ODN, whereas serum IgE levels were decreased by B\u2010type CpG\u2010ODN. We also examined the effects of various ODNs (A, B and C\u2010type CpG\u2010ODNs) and antigens (buckwheat, \u03b1\u2010casein, \u03b2\u2010lactoglobulin and ovalbumin) on anaphylactic severity, and found that the combination of buckwheat and B\u2010type CpG\u2010ODN induced the most intense anaphylactic shock. This model is expected to contribute to the study of the prevention of anaphylactic shock.<\/jats:p>","DOI":"10.1111\/asj.12479","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T01:09:26Z","timestamp":1440464966000},"page":"710-717","source":"Crossref","is-referenced-by-count":6,"title":["Development of a simple IgE\u2010independent anaphylactic model using buckwheat antigen and B\u2010type CpG oligodeoxynucleotide from <i>Streptococcus thermophilus<\/i>"],"prefix":"10.1111","volume":"87","author":[{"given":"Yoshinari","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Interdisciplinary Graduate School of Science and Technology  Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suguru","family":"Shigemori","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate School of Science and Technology  Nagano Japan"},{"name":"Research Fellow of the Japan Society for the Promotion of Science (JSPS)  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