{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T21:41:58Z","timestamp":1774647718735,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T00:00:00Z","timestamp":1480982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We evaluated the effects of mineral salt supplement on enteric methane emissions, ruminal fermentation and methanogen community of dairy cows over a whole lactation period. Ten Holstein cows fed a total mixed ration (TMR) diet were randomly allocated into two groups, one supplied with mineral salts as the treatment group and the other as the control group. The methane measurement showed that the ingestion of mineral salts lowered enteric methane emissions significantly (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), with an average of 10.5% reduction over the whole lactation period. Ruminal fermentation analysis showed the mineral salt intake could significantly decrease the acetate\u00a0:\u00a0propionate ratio (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Real\u2010time PCR assay showed that rumen methanogen abundance significantly reduced in the treatment group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) but was not significantly influenced by mineral salt intake over the whole lactation period. Intergroup methanogen community composition was influenced slightly by mineral salt intake; however, significantly different intragroup profiles were apparent throughout the whole lactation period, according to denaturing gradient gel electrophoresis analysis. In conclusion, these results suggested that the effective mitigation of enteric methane emissions by mineral salt intake could be attributed to decreased density of methanogenic archaea and that fluctuations in methane emission over the lactation period might be related to <jats:italic>Methanobrevibacter<\/jats:italic> diversity.<\/jats:p>","DOI":"10.1111\/asj.12738","type":"journal-article","created":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T05:52:59Z","timestamp":1481003579000},"page":"1049-1057","source":"Crossref","is-referenced-by-count":9,"title":["Effects of mineral salt supplement on enteric methane emissions, ruminal fermentation and methanogen community of lactating cows"],"prefix":"10.1111","volume":"88","author":[{"given":"Xiaohua","family":"Li","sequence":"first","affiliation":[{"name":"Agro\u2010environmental Protection Institute Ministry of Agriculture  Tianjin China"},{"name":"Rural Energy &amp; Environment Agency Ministry of Agriculture  Beijing 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