{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T18:13:58Z","timestamp":1759601638394,"version":"3.41.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T00:00:00Z","timestamp":1484092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Public Security Key Research Program of China","award":["201301ZDYJ005"],"award-info":[{"award-number":["201301ZDYJ005"]}]},{"name":"Ministry of Public Security Application and Innovation Research Program of China","award":["2011YYCXKMJQ167"],"award-info":[{"award-number":["2011YYCXKMJQ167"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to evaluate fertility and sex ratios after artificial insemination in dogs under field conditions. Semen was cryopreserved as unsorted (control) or was separated into X\u2010 and Y\u2010chromosome\u2010bearing sperm using a cell sorter. Sixty female dogs were inseminated with frozen\u2013thawed spermatozoa of 100\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>unsorted (a dose in practice) and 4\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>sorted (X and Y group, respectively). A total of 20 dogs became pregnant and 126 puppies were born from the three groups. The percentage of parturition was similar for the X (5\/20; 25.0%) and Y (4\/20; 20.0%) group (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05), but lower than controls (11\/20; 55.0%) (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Ultimately 28 out of the 32 puppies produced from X group were female (87.5%) and 19\/22 (86.4%) puppies of Y group were male. In contrast, sex ratio (51.4% to 48.6%) in the control was significantly different from the X, Y group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). However, male and female puppies in the control had similar birth weights and weaning weights to those from the X and Y groups. This preliminary information indicated that normal puppies of predicted sex can be produced with low numbers of sorted cryopreserved dog spermatozoa at a farm level, making sperm\u2010sexing technology potentially applicable for elite breeding units.<\/jats:p>","DOI":"10.1111\/asj.12763","type":"journal-article","created":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T06:26:03Z","timestamp":1484202363000},"page":"1232-1238","source":"Crossref","is-referenced-by-count":5,"title":["Birth of puppies of predetermined sex after artificial insemination with a low number of sex\u2010sorted, frozen\u2013thawed spermatozoa in field conditions"],"prefix":"10.1111","volume":"88","author":[{"given":"Yun\u2010Fang","family":"Wei","sequence":"first","affiliation":[{"name":"Kunming Police Dog Base of the Ministry of Public Security Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang\u2010Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Kunming Police Dog Base of the Ministry of Public Security Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010Sheng","family":"Tang","sequence":"additional","affiliation":[{"name":"Kunming Police Dog Base of the Ministry of Public Security Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai\u2010Guo","family":"Mao","sequence":"additional","affiliation":[{"name":"Kunming Police Dog Base of the Ministry of Public Security Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Guang","family":"Li","sequence":"additional","affiliation":[{"name":"Kunming Police Dog Base of the Ministry of Public Security Kunming Yunnan 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