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Each ejaculate was divided into four different groups, as follows: sperm were treated with<jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content>extender (control group) and<jats:styled-content style=\"fixed-case\">TEY<\/jats:styled-content>extender supplemented with three different concentrations of iodixanol (1.25%, 2.50% and 5.00%). Semen straws were frozen in liquid nitrogen vapor. After thawing, sperm motility characteristics, viability, plasma membrane integrity and acrosome integrity were determined. Also, frozen\u2013thawed spermatozoa from all groups were used for<jats:italic>in\u00a0vitro<\/jats:italic>fertilization and artificial insemination (<jats:styled-content style=\"fixed-case\">AI)<\/jats:styled-content>in natural estrus Thai native cows. The results showed that the post\u2010thaw quality of the 2.50% iodixanol group was superior to the other iodixanol groups (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). However, iodixanol had no beneficial effect on post\u2010thaw sperm<jats:italic>in\u00a0vitro<\/jats:italic>fertilization ability and pregnancy rate after<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>(<jats:italic>P\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>0.05). It can be concluded that the supplementation of 2.50% iodixanol extender significantly improves the progressive motility, viability, plasma membrane integrity and acrosome integrity of cryopreserved semen from Thai native bulls, but it has no beneficial effect on<jats:italic>in\u00a0vitro<\/jats:italic>fertilization ability and pregnancy rate after<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/asj.12798","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T05:10:47Z","timestamp":1490937047000},"page":"1311-1320","source":"Crossref","is-referenced-by-count":8,"title":["Effects of supplementation of iodixanol to semen extender on quality and fertilization ability of frozen\u2013thawed Thai native bull sperm"],"prefix":"10.1111","volume":"88","author":[{"given":"Phirawit","family":"Chuawongboon","sequence":"first","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture Khon Kaen 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