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To test this hypothesis, we examined the coexpression of GnRHR, GPR153, and either luteinizing hormone or follicle\u2010stimulating hormone in AP tissue and cultured AP cells by immunofluorescence microscopy. GPR153 was detected in the gonadotrophs, and was colocalized with GnRHR in the plasma membrane. GPR153 was also detected in the cytoplasm of cultured gonadotrophs. Real\u2010time PCR and western blot analyses found that expression was lower (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) in AP tissues during early luteal phase as compared to pre\u2010ovulation or late luteal phases. The 5\u2032\u2010flanking region of the <jats:italic>GPR153<\/jats:italic> gene contained a consensus response element sequence for estrogen, but not for progesterone. These data suggest that some, but not all GPR153 colocalizes with GnRHR in the plasma membrane of gonadotrophs, and its expression changes stage\u2010dependently in the bovine AP.<\/jats:p>","DOI":"10.1111\/asj.12920","type":"journal-article","created":{"date-parts":[[2017,9,27]],"date-time":"2017-09-27T08:43:24Z","timestamp":1506501804000},"page":"60-71","source":"Crossref","is-referenced-by-count":3,"title":["Heifers express G\u2010protein coupled receptor 153 in anterior pituitary gonadotrophs in stage\u2010dependent manner"],"prefix":"10.1111","volume":"89","author":[{"given":"Kiran","family":"Pandey","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Yamaguchi University Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onalenna","family":"Kereilwe","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Yamaguchi University Yamaguchi 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