{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:52:48Z","timestamp":1790009568111,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T00:00:00Z","timestamp":1514419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Coccidiosis is an intestinal parasitic infection and one of the most prevalent and economically damaging diseases of chickens. Furthermore, coccidia\u2010induced mucogenesis promotes secondary colonization by <jats:italic>Clostridium perfringens<\/jats:italic>, a major pathogen of chickens that causes necrotic enteritis. Our previous work found that supernatant of a culture of <jats:italic>Bacillus amyloliquefaciens<\/jats:italic> strain <jats:styled-content style=\"fixed-case\">TOA<\/jats:styled-content>5001 (<jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>) inhibited the growth of <jats:italic>C. perfringens<\/jats:italic> on Gifu anaerobic broth medium. Accordingly, we evaluated the effectiveness of dietary <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content> administration in inhibiting <jats:italic>C. perfringens<\/jats:italic> colonization of the intestine in broilers that were experimentally infected with coccidia. Ten healthy broilers from a <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>\u2010supplemented (2\u00a0\u00d7\u00a010<jats:sup>5<\/jats:sup>\u00a0colony\u2010forming units\/g of feed) broiler group and 10 from a non\u2010treated group were challenged with <jats:italic>Eimeria tenella<\/jats:italic> and <jats:italic>E. maxima<\/jats:italic> (5000 oocysts of each species\/chick) at 28 days old. At 36 days old, five chicks from each group were slaughtered, whereas the remaining five in each group were killed at 49 days old. Dietary <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content> administration into <jats:italic>Eimeria<\/jats:italic>\u2010challenged birds reduced coccidial symptoms such as intestinal lesions. It also modified the cecal microbiota through suppressing <jats:italic>C. perfringens<\/jats:italic> and <jats:italic>E.\u00a0coli<\/jats:italic> colonization, and inducing domination of <jats:italic>Faecalibacterium prausnitzii<\/jats:italic>, the <jats:italic>Lactobacillus<\/jats:italic> group and unknown Lachnospiraceae genera by bacterial <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010based metagenome analyses. <jats:italic>B. amyloliquefaciens <\/jats:italic><jats:styled-content style=\"fixed-case\">TOA<\/jats:styled-content>5001 supplementation suppressed the symptoms of coccidiosis by modulating cecal microbiota in <jats:italic>Eimeria<\/jats:italic>\u2010challenged broilers.<\/jats:p>","DOI":"10.1111\/asj.12980","type":"journal-article","created":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T07:42:45Z","timestamp":1514446965000},"page":"679-687","source":"Crossref","is-referenced-by-count":44,"title":["Inclusion of <i>Bacillus amyloliquefaciens<\/i> strain <scp>TOA<\/scp>5001 in the diet of broilers suppresses the symptoms of coccidiosis by modulating intestinal microbiota"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3902-5956","authenticated-orcid":false,"given":"Takamitsu","family":"Tsukahara","sequence":"first","affiliation":[{"name":"Kyoto Institute of Nutrition &amp; Pathology Ujitawara Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7233-366X","authenticated-orcid":false,"given":"Ryo","family":"Inoue","sequence":"additional","affiliation":[{"name":"Kyoto Prefectural University Shimogamo Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keizo","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Kyoto Institute of Nutrition &amp; Pathology Ujitawara Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takio","family":"Inatomi","sequence":"additional","affiliation":[{"name":"TOA Pharmaceutical Co. Ltd. Tokyo Japan"},{"name":"Inatomi Animal Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03718"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782009005000135"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.1.62"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.3.446"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr381"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-01993"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00484"},{"key":"e_1_2_5_11_1","first-page":"2","article-title":"Poultry coccidiosis: Prevention and control approaches","volume":"72","author":"Hafez MH","year":"2008","journal-title":"Archiv f\u00fcr Gefl\u00fcgelkunde"},{"key":"e_1_2_5_12_1","first-page":"33","article-title":"Morphometric and histopathological evaluation of a probiotic and its synergism with vaccination against coccidiosis in broilers","volume":"1","author":"Hayakawa T","year":"2014","journal-title":"Animal Science Letters"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4423-4427.2002"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3164\/jcbn.17-44","article-title":"Prediction of functional profiles of gut microbiota from 16S rRNA metagenomic data provides a more robust evaluation of gut dysbiosis occurring in Japanese type 2 diabetic patients","volume":"61","author":"Inoue R","year":"2017","journal-title":"Journal of Clinical Biochemistry and Nutrition"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(70)90063-9"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2014.09.004"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/86.1.63"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2010.06.001"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.14.0330"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0712038105"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pev124"},{"key":"e_1_2_5_22_1","doi-asserted-by":"crossref","first-page":"689","DOI":"10.3923\/ijps.2013.689.697","article-title":"Pyrosequencing analysis of salinomycin and Eimeria spp. challenge\u2010induced changes in broiler cecal microbial communities","volume":"12","author":"Martynova\u2010Van Kley A","year":"2013","journal-title":"International Journal of Poultry Science"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0377-8401(03)00163-9"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2006.06.001"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11467.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.3382\/japr.2013-00742"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02409.x"},{"key":"e_1_2_5_28_1","unstructured":"SatoN AbeM SasakiK.2016.Bacillus amyloliquefaciens strain TOA5001 and antibacterial composition within its strain. Japanese Patent Publication No. 2016\u2010116466 granted 30 June 2016."},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.05.021"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.2307\/1590289"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4185-4190.2005"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.49.414"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.09-0406"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12003"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-0929.2012.01039.x"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00086-7"},{"key":"e_1_2_5_37_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.vetpar.2005.11.004","article-title":"Relative virulences of a drug\u2010resistant and a drug\u2010sensitive strain of Eimeria acervulina, a coccidium of chickens","volume":"135","author":"Williams RB","year":"2006","journal-title":"Veterinary Parasitology"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3911-3916.2005"}],"container-title":["Animal Science Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fasj.12980","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.12980","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T11:26:37Z","timestamp":1695554797000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/asj.12980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,28]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/asj.12980"],"URL":"https:\/\/doi.org\/10.1111\/asj.12980","archive":["Portico"],"relation":{},"ISSN":["1344-3941","1740-0929"],"issn-type":[{"value":"1344-3941","type":"print"},{"value":"1740-0929","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,28]]}}}