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Animals were divided into four groups, each composed of seven cows, depending on the i.u. treatment used: Group A\u2014cephapirin; Group B\u2014methisoprinol; Group C\u2014cephapirin and methisoprinol at the same time; and a control group\u2014without medication. Using flow cytometry technique, the phagocytic activity of granulocytes and monocytes was identified, as well as the oxidative burst activity of neutrophils in the peripheral blood and uterine washings. Summarizing the results of the research, i.u. infusion of cephapirin caused a reduction in the phagocytic and killing activity of phagocytes. The i.u. use of methisoprinol increased phagocytic and killing activity of phagocytes in the uterus. Administering both listed substances simultaneously showed a decrease in phagocytosis, presumably due to the dominating inhibitor effect of the antibiotic. However, also an increase of mean fluorescence intensity was observed, presumably caused by the methisoprinol. Intrauterine use of immunostimulatory substances, can improve the effectiveness of the treatment of endometritis in cows.<\/jats:p>","DOI":"10.1111\/asj.13088","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T03:08:31Z","timestamp":1534475311000},"page":"1389-1397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Phagocytic and oxidative burst activity of phagocytic cells in peripheral blood and uterine washings in cows with clinical endometritis before and after intrauterine use of cephapirin and methisoprinol"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0809-2101","authenticated-orcid":false,"given":"Piotr","family":"Brodzki","sequence":"first","affiliation":[{"name":"Department and Clinic of Animal Reproduction Faculty of Veterinary Medicine University of Life Sciences in Lublin Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urszula","family":"Lisiecka","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Infections Diseases Faculty of Veterinary Medicine University of Life Sciences in Lublin Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Brodzki","sequence":"additional","affiliation":[{"name":"Department and Clinic of Animal Surgery Faculty of Veterinary Medicine University of Life Sciences in Lublin Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Pyz\u2010\u0141ukasik","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene of Animal Origin Faculty of Veterinary Medicine University of Life Sciences in Lublin Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leszek","family":"Krakowski","sequence":"additional","affiliation":[{"name":"Department and Clinic of Animal Reproduction Faculty of Veterinary Medicine University of Life Sciences in Lublin Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2010000500002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0203082"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2010-3550"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2013.10.025"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2001.13.6.615"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(01)00124-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2885.1998.00159.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.08.006"},{"key":"e_1_2_6_10_1","first-page":"290","article-title":"Postpartum uterine diseases in dairy cows","volume":"9","author":"Galv\u00e3o K. 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