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Transmissible gastroenteritis virus (<jats:styled-content style=\"fixed-case\">TGEV<\/jats:styled-content>) is main pathogens responsible for piglets viral diarrhea. Knockout the host cellular surface receptor for <jats:styled-content style=\"fixed-case\">TGEV<\/jats:styled-content> may be an effective way to accelerate the breeding of resistant pigs. In this study, we applied site\u2010specific editing <jats:styled-content style=\"fixed-case\">pAPN<\/jats:styled-content> which is effective in swine testis (<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>) cells. Site\u2010specific editing of <jats:styled-content style=\"fixed-case\">pAPN<\/jats:styled-content> reduced <jats:styled-content style=\"fixed-case\">TGEV<\/jats:styled-content> proliferation in <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content> cells by 96%\u201399% at different time periods post\u2010infection. Next, the site\u2010specific editing of <jats:styled-content style=\"fixed-case\">pAPN<\/jats:styled-content> porcine fetal fibroblasts were produced, and then the cell colonies were used as donor cells to generate the site\u2010specific editing of <jats:styled-content style=\"fixed-case\">pAPN<\/jats:styled-content> pigs. Our research findings will not only offer a more thorough understanding of the pathogenesis of piglet diarrhea and lay the foundation for breeding <jats:styled-content style=\"fixed-case\">TGEV<\/jats:styled-content>\u2010resistant piglets, but also understanding the molecular mechanisms involved in coronaviral infections.<\/jats:p>","DOI":"10.1111\/asj.13163","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T07:54:52Z","timestamp":1547020492000},"page":"366-371","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Production of porcine aminopeptidase N (<i><scp>pAPN<\/scp><\/i>) site\u2010specific edited pigs"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1904-2676","authenticated-orcid":false,"given":"Jianwen","family":"Chen","sequence":"first","affiliation":[{"name":"Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding College of Animal Science and Technology Anhui Agricultural University  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiyuan","family":"Pan","sequence":"additional","affiliation":[{"name":"Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding College of Animal Science and Technology Anhui Agricultural University  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding College of Animal Science and Technology Anhui Agricultural University  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4661-9453","authenticated-orcid":false,"given":"Yunsheng","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding College of Animal Science and Technology Anhui Agricultural University  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biao","family":"Ding","sequence":"additional","affiliation":[{"name":"Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding College of Animal Science and Technology Anhui Agricultural University  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyang","family":"Han","sequence":"additional","affiliation":[{"name":"Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding College of Animal Science and Technology Anhui Agricultural University  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zubing","family":"Cao","sequence":"additional","affiliation":[{"name":"Anhui 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