{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T21:28:58Z","timestamp":1775597338569,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T00:00:00Z","timestamp":1564963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Shaanxi Province Special Fund Project","award":["NYKJ-2018-YL01"],"award-info":[{"award-number":["NYKJ-2018-YL01"]}]},{"name":"Xi'an Science and Technology Plan Project","award":["201806115YF03NC11(2)"],"award-info":[{"award-number":["201806115YF03NC11(2)"]}]},{"name":"Shaanxi Province Technology Innovation Guide Special Project","award":["2018ZKC-184"],"award-info":[{"award-number":["2018ZKC-184"]}]},{"name":"National Key R&D Program of China","award":["2018YFD501000"],"award-info":[{"award-number":["2018YFD501000"]}]},{"name":"National Pig Industry Technology System of China","award":["CARS-36"],"award-info":[{"award-number":["CARS-36"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sulfanilamide (SA) is an effective broad\u2010spectrum antibacterial agent in human and veterinary medicine. The purpose of this study was to evaluate the effects of SA on boar sperm quality during liquid storage at 17\u00b0C and determine the optimal concentration of SA and its effects on bacterial growth, microbial composition, and maternal fertility. Boar ejaculates were diluted with a basic extender, containing different concentrations of SA, and stored in a 17\u00b0C incubator for 6\u00a0days. The sperm motility, plasma membrane integrity, and acrosome integrity were measured daily. The results showed that when the concentration of SA was 0.02\u00a0g\/L, the sperm quality parameters were significantly higher than those of all other treatment groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). We also monitored the bacterial growth and compared the differences in the microbial species between the 0.02\u00a0g\/L SA group and the control by 16S rDNA sequencing. The results revealed that some bacteria, such as <jats:italic>Staphylococcus<\/jats:italic> and <jats:italic>Pseudomonas<\/jats:italic>, were considerably lower in the 0.02\u00a0g\/L SA group than in the control group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In addition, preserved semen was used for artificial insemination, and results showed that 0.02\u00a0g\/L SA group had a higher litter size, and its pregnancy rate was 92.5%.<\/jats:p>","DOI":"10.1111\/asj.13281","type":"journal-article","created":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T13:18:37Z","timestamp":1565011117000},"page":"1161-1169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17\u00b0C"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0284-6339","authenticated-orcid":false,"given":"Tian\u2010Yu","family":"Feng","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fa","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gui\u2010Chao","family":"Dai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua\u2010Ming","family":"Xi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang\u2010Yi","family":"Hao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Hong","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00261-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.031"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12555"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/01932690701817966"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4081\/ijas.2014.3082"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.11.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.07.018"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/173637a0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2013.787422"},{"issue":"4","key":"e_1_2_7_11_1","first-page":"23","article-title":"Bacterial and fungal burden in boar semen","volume":"7","author":"Ciornei S. G.","year":"2012","journal-title":"Research Journal of Biotechnology"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2015.04.037"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13024"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.13.97"},{"issue":"1","key":"e_1_2_7_15_1","first-page":"153","article-title":"Effect of a surfactant on the integrity of frozen ram spermatozoa","volume":"29","author":"Hellemann C.","year":"1997","journal-title":"Archivos de Medicina Veterinaria"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.03.015"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.10.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.09.049"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12865"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.08.047"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.02.003"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.08.002"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.5433\/1679-0359.2013v34n1p341"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2006.08.003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.05.019"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/08997659.2014.966211"},{"issue":"5","key":"e_1_2_7_27_1","first-page":"291","article-title":"Function of the antioxidant system in boar semen","volume":"72","author":"Orzolek A.","year":"2016","journal-title":"Medycyna Weterynaryjna\u2010Veterinary Medicine\u2010Science and Practice"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.05.008"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.01.014"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2018.08.002"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2016.07.006"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2018.08.006"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.03.035"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12921"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00108-09"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105949"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2016.08.032"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.2174\/157341210791936803"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2010.02.042"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.02.011"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/sky372"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.08.006"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22840"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12545"}],"container-title":["Animal Science Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fasj.13281","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.13281","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/asj.13281","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.13281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T22:57:54Z","timestamp":1694300274000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/asj.13281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,5]]},"references-count":43,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1111\/asj.13281"],"URL":"https:\/\/doi.org\/10.1111\/asj.13281","archive":["Portico"],"relation":{},"ISSN":["1344-3941","1740-0929"],"issn-type":[{"value":"1344-3941","type":"print"},{"value":"1740-0929","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,5]]},"assertion":[{"value":"2019-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}