{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:21:16Z","timestamp":1767165676111,"version":"build-2238731810"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Embryo transfer (ET) of 20 porcine expanded blastocysts (ExBs) vitrified and warmed (VW) by open pulled straw (OPS) to a recipient allows stable piglet production. The efficiency of artificial insemination (AI) prior to ET of 10 VW ExBs for piglet production was investigated. For one trial, 10\u201315 VW ExBs from single donor were assigned, 10 were used for ET and the remains were assessed for their in vitro viability. In the non\u2010AI\/ET group, 10 were transferred to each of five recipients. As AI\/ET group, 10 were transferred to each of five recipients after AI. In AI\/non\u2010ET group, only AI was performed to seven gilts. In the non\u2010AI\/ET group, the pregnancy rate was 40%, but none of them farrowed. In the AI\/ET group, all recipients produced piglets. Four (80.0%) delivered piglets from transferred VW ExBs. The survival rate of VW ExBs to term was 20.0% (10\/50). In the AI\/non\u2010ET group, six of the seven gilts farrowed. There was no difference in in vitro viability between the non\u2010AI\/ET and AI\/ ET groups (62.5% and 68.3%, respectively). AI prior to ET can be an appropriate way to maintain pregnancy and assist the development of a low number of VW ExBs to term.<\/jats:p>","DOI":"10.1111\/asj.13325","type":"journal-article","created":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T23:24:45Z","timestamp":1577402685000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The effect of artificial insemination prior to transfer of a limited number of vitrified and warmed porcine embryos by open pulled straw (OPS) method on their survival ability for farrowing"],"prefix":"10.1111","volume":"91","author":[{"given":"Shigeyuki","family":"Tajima","sequence":"first","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenzo","family":"Uchikura","sequence":"additional","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Kurita","sequence":"additional","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7198-9237","authenticated-orcid":false,"given":"Kazuhiro","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences Institute of Agrobiological Sciences National Agriculture and Food Research Organization (NARO) Tsukuba Japan"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00720-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.12.014"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.2000.2273"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2001129"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.04.050"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2527\/1991.6983335x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.04.023"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1989.tb09714.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0860679"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093-691x(03)00257-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.12.018"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.3.564"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.08.008"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.05.045"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.17076"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5178"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.03.022"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1230507"},{"key":"e_1_2_6_20_1","first-page":"251","article-title":"Effective concentration of ethylene glycol for cryoprotectant on vitrification of embryo","volume":"33","author":"Kobayashi S.","year":"2001","journal-title":"Research Bulletin of the Aichi\u2010ken Agricultural Research Center"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.1997.2056"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2013-045"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093-691x(02)01364-x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.06.024"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0120395"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1030331"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod35.1.133"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006-578"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00246-6"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.50.481"},{"key":"e_1_2_6_31_1","first-page":"231","volume-title":"Boar Semen Preservation II, Proceedings Second International Conference on Boar Semen Preservation","author":"Weitze K. 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