{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:55:59Z","timestamp":1762520159704,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T00:00:00Z","timestamp":1591833600000},"content-version":"vor","delay-in-days":162,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was aimed to explore the comparative acidifying properties of 2\u2010hydroxy\u20104\u2010(methylthio) butanoic acid (HMTBA) and a combination of DL\u2010methionine (DLM) and acidifier in male broiler production. A total of 480 1\u2010day\u2010old broiler chicks were randomly divided into four treatments: A (low HMTBA, 0.057% HMTBA); B (low acidifier, 0.05% DLM\u00a0+\u00a00.057% acidifier); C (high HMTBA, 0.284% HMTBA); and D (high acidifier, 0.25% DLM\u00a0+\u00a00.284% acidifier). At 21 d, growth performance, chyme pH, digestive enzyme activities, and intestinal microflora were measured. The pH of crop, gizzard, and ileum contents was higher in the HMTBA treatment group than in DLM\u00a0+\u00a0acidifier treatment group. Furthermore, acidifier supplementation promoted growth of butyrate\u2010producing bacteria such as <jats:italic>Faecalibacterium<\/jats:italic>, whereas high HMTBA (0.284%) inhibited the proliferation of acid\u2010producing bacteria including <jats:italic>Roseburia<\/jats:italic> and <jats:italic>Collinsella<\/jats:italic>. The chymotrypsin activity was lower in the HMTBA group than in the DLM\u00a0+\u00a0acidifier group. In contrast, high\u2010level HMTBA group showed higher average daily gain and average daily feed intake than the DLM\u00a0+\u00a0acidifier group. These results suggested that HMTBA work through different pathways with DLM plus acidifier.<\/jats:p>","DOI":"10.1111\/asj.13409","type":"journal-article","created":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T07:46:57Z","timestamp":1591861617000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effect of 2\u2010hydroxy\u20104\u2010(methylthio) butanoic acid and acidifier on the performance, chyme pH, and microbiota of broilers"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1762-1896","authenticated-orcid":false,"given":"Yuqin","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaonan","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youli","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahir","family":"Mahmood","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Shahid","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dafei","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianmin","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pev340"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0396.1988.tb00057.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps092205"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00271\u201010"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2015.09.019"},{"key":"e_1_2_6_8_1","unstructured":"Geraert P. Graulet P. Mercier Y. &Becker P.&van derKlisJ..(2005)The bactericidal effect of methionine hydroxy analog. In: Proceedings of the 17th Australian Poultry Science Symposium Sydney New South Wales Australia 7\u20139 February 2005 pp. 246\u2013247. Poultry Research Foundation."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.17533\/udea.rccp.v28n2a01"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.17221\/8077-CJAS"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.32"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2676"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex082"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.06.001"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skz348"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03046\u201012"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201013\u201031"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177735"},{"volume-title":"Nutrient requirements of poultry: 1994","year":"1994","author":"National Research Council","key":"e_1_2_6_19_1"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369\u2010703X(01)00102\u20104"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1071\/AN15061"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01046"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1988.66102598x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801193105"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/17450390701556817"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/84.9.1397"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex232"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.21071\/az.v64i245.375"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9285"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021989"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102011\u201012\u20106\u2010r60"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05391\u201011"},{"volume-title":"SPSS Base 20.0. SPSS","year":"2010","author":"SPSS software","key":"e_1_2_6_33_1"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933917000551"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.8.1409"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00062-07"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26818"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198985"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/1745039X.2015.1112622"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2015.1021296"}],"container-title":["Animal Science Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.13409","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/asj.13409","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.13409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T15:46:36Z","timestamp":1692719196000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/asj.13409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/asj.13409"],"URL":"https:\/\/doi.org\/10.1111\/asj.13409","archive":["Portico"],"relation":{},"ISSN":["1344-3941","1740-0929"],"issn-type":[{"type":"print","value":"1344-3941"},{"type":"electronic","value":"1740-0929"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13409"}}