{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T06:35:47Z","timestamp":1767162947763,"version":"build-2238731810"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"vor","delay-in-days":257,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study was performed to evaluate reproductive performance after non\u2010surgical embryo transfer (Ns\u2010ET) of 10\u201315 porcine expanded blastocysts (ExBs) that had been vitrified and warmed (V\/W) using the micro volume air cooling (MVAC) method. The effect of asynchrony between the donor and recipient estrous cycle was investigated. Ns\u2010ET was conducted in recipients whose estrous cycle was asynchronous to that of donors by a delay of 2, 1, or 0\u00a0days. In the 2\u2010day and 1\u2010day groups, the similar farrowing rates (27.3% and 25.0%) and survival rates to term (13.9% and 15.7%) were obtained after Ns\u2010ET of V\/W ExBs. None of the recipients in 0\u2010day group farrowed. Artificial insemination (AI) prior to Ns\u2010ET was then evaluated. Ten\u201315\u00a0V\/W ExBs were transferred non\u2010surgically to 12 recipients whose estrous cycles were asynchronous to that of donors by a 2\u2010day delay. All of the recipients produced piglets, and all (100.0%) delivered piglets were derived from the transferred V\/W ExBs. The survival rate of V\/W ExBs to term was 25.2%. These results demonstrate that Ns\u2010ET of V\/W ExBs using MVAC can facilitate piglet production, even if 10\u201315 embryos are transferred. Moreover, piglets were obtained stably when AI was performed prior to Ns\u2010ET.<\/jats:p>","DOI":"10.1111\/asj.13453","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T23:22:02Z","timestamp":1600730522000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Insemination of recipient sows improves the survival to term of vitrified and warmed porcine expanded blastocysts transferred non\u2010surgically"],"prefix":"10.1111","volume":"91","author":[{"given":"Shigeyuki","family":"Tajima","sequence":"first","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenzo","family":"Uchikura","sequence":"additional","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Kurita","sequence":"additional","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7198-9237","authenticated-orcid":false,"given":"Kazuhiro","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences Institute of Agrobiological Sciences National Agriculture and Food Research Organization (NARO) Tsukuba Japan"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.05.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2014\u2010022"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(01)00720\u20108"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.2000.2273"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2001129"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2527\/1991.6983335x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2004.04.039"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33915"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.3.564"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.05.045"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.17076"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.05.035"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(00)00252\u20101"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.1997.2056"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.2.451"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(03)00190\u20100"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10587"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2013\u2010045"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(02)01364\u2010x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.06.024"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.5938\/youton.52.1"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0120395"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.11.008"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13325"},{"key":"e_1_2_7_26_1","first-page":"231","volume-title":"Boar Semen Preservation II, Proceedings Second International Conference on Boar Semen Preservation","author":"Weitze K. 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