{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:47:45Z","timestamp":1790876865901,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T00:00:00Z","timestamp":1603065600000},"content-version":"vor","delay-in-days":292,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007172","name":"National Agriculture and Food Research Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007172","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the feasibility of piglet production by non\u2010surgical embryo transfer (Ns\u2010ET) of vitrified porcine blastocysts and expanded blastocysts transported to commercial farms and warmed on site (V\/T\/W embryos). Ns\u2010ET was performed by depositing 11\u201320 vitrified and warmed embryos at a proximal site within the uterus via a catheter. In Experiment 1, the effect of donor\u2013recipient estrous cycle asynchrony on the efficiency of Ns\u2010ET of vitrified and ordinary warmed embryos was investigated at the experimental facility. With a 1\u2010day delay recipients relative to that of donor, the farrowing rate was 50.0% and the survival rate to term was 21.1%. In Experiment 2, Ns\u2010ET using recipients with a 1\u2010day delay and vitrified embryos after one\u2010step warming and dilution was evaluated at the experimental facility. Although the resulting farrowing rate was 42.9%, the survival rate was 6.4%. In Experiment 3, Ns\u2010ET was conducted using V\/T\/W embryos at four commercial farms, where piglets derived from them were produced. When artificial insemination was conducted prior to Ns\u2010ET, the farrowing and survival rates obtained using V\/T\/W embryos were 75.0%, and 21.3%, respectively. These results show that Ns\u2010ET of V\/T\/W embryos using this protocol would be feasible for piglet production at farms.<\/jats:p>","DOI":"10.1111\/asj.13476","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T07:01:16Z","timestamp":1603177276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Piglet production by non\u2010surgical transfer of vitrified embryos, transported to commercial swine farms and warmed on site"],"prefix":"10.1111","volume":"91","author":[{"given":"Shigeyuki","family":"Tajima","sequence":"first","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sawako","family":"Motoyama","sequence":"additional","affiliation":[{"name":"Saga Prefectural Livestock Experiment Station Takeo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Wakiya","sequence":"additional","affiliation":[{"name":"Saga Prefectural Livestock Experiment Station Takeo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenzo","family":"Uchikura","sequence":"additional","affiliation":[{"name":"Aichi Agricultural Research Center Nagakute Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyasu","family":"Misawa","sequence":"additional","affiliation":[{"name":"First development Gr. 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Ltd Kasama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"Takishita","sequence":"additional","affiliation":[{"name":"National Livestock Breeding Center Miyazaki Station Miyazaki Kobayashi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2328-8417","authenticated-orcid":false,"given":"Yuri","family":"Hirayama","sequence":"additional","affiliation":[{"name":"Department of Planning and Coordination National Livestock Breeding Center Fukushima Nishishirakawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7198-9237","authenticated-orcid":false,"given":"Kazuhiro","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences Institute of Agrobiological Sciences National Agriculture and Food Research Organization (NARO) Tsukuba Japan"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.05.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2014\u2010022"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2527\/1991.6983335x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2004.04.039"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.12.018"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33915"},{"key":"e_1_2_7_8_1","first-page":"57","article-title":"Effect of migration, distribution and spacing of pig embryos on pregnancy and fetal survival","volume":"33","author":"Dziuk P.","year":"1985","journal-title":"Journal of Reproduction and Fertility"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.05.045"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.05.035"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(00)00252\u20101"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13457"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.2.451"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(03)00190\u20100"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10587"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.5938\/youton.57.4_129"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2013\u2010045"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.06.024"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.19132"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0120395"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13325"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13453"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.5938\/youton.57.4_138"},{"key":"e_1_2_7_24_1","first-page":"231","volume-title":"Boar Semen Preservation II, Proceedings Second International Conference on Boar Semen Preservation","author":"Weitze K. 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