{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:50:16Z","timestamp":1788486616076,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T00:00:00Z","timestamp":1607731200000},"content-version":"vor","delay-in-days":346,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although successful fertilization is completed by only 150 sperm in the pig oviduct, more than 50,000 sperms are required to achieve a fertilization rate of more than 70% by pig in vitro fertilization (IVF). In this study, to improve the efficiency of pig IVF, the effects of hypoxic conditions and treatment with creatine and methyl\u2010beta cyclodextrin (M\u03b2CD) on the glycolytic pathway were investigated. Under low O<jats:sub>2<\/jats:sub> conditions, zig\u2010zag motility was strongly induced within 30\u00a0min; however, the induction disappeared at 60\u00a0min. Although caffeine suppressed zig\u2013zag motility under low O<jats:sub>2<\/jats:sub> conditions, creatine induced and sustained zig\u2013zag motility until 120\u00a0min. Additionally, pretreatment with M\u03b2CD for 15\u00a0min greatly enhanced zig\u2013zag motility via ATP production in sperm incubated with creatine under low O<jats:sub>2<\/jats:sub> conditions. Sperm pretreated with M\u03b2CD were used for IVF in medium containing creatine under low O<jats:sub>2<\/jats:sub> conditions. A fertilization rate of approximately 70% was achieved with only 1.0 x 10<jats:sup>4<\/jats:sup> sperms\/mL, and there were few polyspermic embryos. Therefore, our novel method was beneficial for efficient production of pig embryos in vitro. Moreover, the zig\u2013zag motility may be a novel movement which boar capacitated sperm exhibit in the culture medium.<\/jats:p>","DOI":"10.1111\/asj.13493","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T04:37:02Z","timestamp":1607834222000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Methyl\u2010beta cyclodextrin and creatine work synergistically under hypoxic conditions to improve the fertilization ability of boar ejaculated sperm"],"prefix":"10.1111","volume":"91","author":[{"given":"Takashi","family":"Umehara","sequence":"first","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University Higashi\u2010Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsumi","family":"Tsujita","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University Higashi\u2010Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Goto","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University Higashi\u2010Hiroshima Japan"},{"name":"Livestock Research Institute Oita Prefectural Agriculture, Forestry and Fisheries Research Center Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Tonai","sequence":"additional","affiliation":[{"name":"Graduate School of Comprehensive Scientific Research Prefectural University of Hiroshima Shobara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoya","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Graduate School of Comprehensive Scientific Research Prefectural University of Hiroshima Shobara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhisa","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Graduate School of Comprehensive Scientific Research Prefectural University of Hiroshima Shobara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6500-2088","authenticated-orcid":false,"given":"Masayuki","family":"Shimada","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University Higashi\u2010Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(97)00270\u20107"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2009.10.012"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.178.4060.455"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei350"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041246"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.12.7560"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2012.08.018"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008\u2010682X.135123"},{"key":"e_1_2_7_10_1","first-page":"527","article-title":"Anabiosis in boar spermatozoa","volume":"27","author":"Feredean T.","year":"1970","journal-title":"Lucrarile Stiintifice Ale Institutul De Cercetari Zootehnice"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.48.57"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0990097"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098\u20102795"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(03)00209\u20107"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.05.145"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20099"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0540379"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005\u20102728(94)90058\u20102"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202487200"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165\u20100378(01)00098\u20105"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104320(96)01631\u20104"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407708101"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00313.2002"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.026054"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.39.347"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0700271"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0960699"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093\u2010691X(88)90308\u20101"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.138982"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2013.04.071"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.53.2.561"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1398"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.05.045"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)31580-6"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod46.3.419"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.6.1280"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod46.4.686"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod44.2.375"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmi047"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.12.3092"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey081"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20351"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/JBC.274.5.3235"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00252"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.07.008"}],"container-title":["Animal Science Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.13493","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/asj.13493","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.13493","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T15:46:38Z","timestamp":1692719198000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/asj.13493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/asj.13493"],"URL":"https:\/\/doi.org\/10.1111\/asj.13493","archive":["Portico"],"relation":{},"ISSN":["1344-3941","1740-0929"],"issn-type":[{"value":"1344-3941","type":"print"},{"value":"1740-0929","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2020-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13493"}}