{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:23:53Z","timestamp":1788891833723,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T00:00:00Z","timestamp":1610841600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The objective of this study was to investigate the individual and combined effects of essential oils (EO; comprised of thymol and carvacrol) and lysozyme on experimental NE in broiler chickens. A total of 320 1\u2010day\u2010old chicks were randomly assigned to five treatment groups: no\u2010challenge control (NC), NC\u00a0+\u00a0\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>perfringens<\/jats:italic>\n                    challenge (CC), CC\u00a0+\u00a0120\u00a0mg\/kg of EO, CC\u00a0+\u00a0100\u00a0mg\/kg of lysozyme, and CC\u00a0+\u00a0120\u00a0mg\/kg of EO\u00a0+\u00a0100\u00a0mg\/kg of lysozyme. The results showed that EO or lysozyme decreased the mortality, alleviated the gut lesions, inhibited the liver\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    carriage, and increased the villus height of the ileum compared with CC (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05), although the proliferation of\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>perfringens<\/jats:italic>\n                    in the ileum was not inhibited (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a0.05). Moreover, EO or lysozyme was found to decrease the ileal concentration of sialic acid and the\n                    <jats:italic>Mucin2<\/jats:italic>\n                    mRNA expression (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). However, the blend of EO and lysozyme did not display significant effect on the NE\u2010associated mortality or gut damage in contrast with CC (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a0.05). In conclusion, these findings suggest the similar protective effects of EO and lysozyme in NE\u2010associated mortality and intestinal impairment, but their blend did not exhibit ameliorative effect.\n                  <\/jats:p>","DOI":"10.1111\/asj.13499","type":"journal-article","created":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T05:24:52Z","timestamp":1610861092000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Dietary supplementation of essential oils and lysozyme reduces mortality and improves intestinal integrity of broiler chickens with necrotic enteritis"],"prefix":"10.1111","volume":"92","author":[{"given":"Encun","family":"Du","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing P. R. China"},{"name":"Hubei Key Laboratory of Animal Embryo and Molecular Breeding Institute of Animal Science and Veterinary Medicine Hubei Academy of Agricultural Sciences Wuhan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8577-4803","authenticated-orcid":false,"given":"Yuming","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing P. R. 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