{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:46:29Z","timestamp":1788367589730,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T00:00:00Z","timestamp":1616630400000},"content-version":"vor","delay-in-days":83,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The objective of this study was to use luteinizing hormone\u2010releasing hormone A3 (LRH\u2010A3) and human chorionic gonadotrophin (HCG) to improve pregnancy rate of dairy cows during timed artificial insemination (TAI). In experiment 1, the TAI process (0\u00a0d, GnRH, 100\u00a0\u03bcg; 7\u00a0d, PGF2\u03b1, 0.4\u00a0mg; 56\u00a0hr, GnRH, 100\u00a0\u03bcg; 16\u00a0hr, AI) was applied to 160 dairy cows on 50th and 60th days after parturition respectively. In experiment 2, 320 postpartum dairy cows were treated with TAI (Group A), TAI\u00a0+\u00a025\u00a0\u03bcg LRH\u2010A3 (Group B), TAI\u00a0+\u00a01,500\u00a0IU HCG 5\u00a0days after AI (Group C), and TAI\u00a0+\u00a025\u00a0\u03bcg LRH\u2010A3\u00a0+\u00a01,500\u00a0IU HCG 5\u00a0days after AI (Group D). In experiment 3, endometrial cells were treated with HCG. The results showed that TAI did not affect the pregnancy rate, while LRH\u2010A3 and HCG increased the pregnancy rate of the cow. HCG of 5\u00a0IU\/ml and 10\u00a0IU\/ml increased the expressions of leukemia inhibitory factor but decreased those of interleukin\u20106, epidermal growth factor and vascular endothelial growth factor in endometrial cells. This study provided a plan for the use of LRH\u2010A3 and HCG to increase pregnancy rate during TAI in dairy cows.<\/jats:p>","DOI":"10.1111\/asj.13549","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T04:21:23Z","timestamp":1616646083000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["LRH\u2010A3 and HCG increase pregnancy rate during timed artificial insemination in dairy cows"],"prefix":"10.1111","volume":"92","author":[{"given":"Peng","family":"Zheng","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4442-5652","authenticated-orcid":false,"given":"He","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University Harbin 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