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Accumulation of adipocytes is also observed under the pathological condition of skeletal muscle such as muscular dystrophy and sarcopenia. The origin of adipocytes seen in skeletal muscle is mesenchymal progenitor cells that can give rise to both adipocytes and fibroblasts. In the present study, we demonstrated that siRNA\u2010mediated suppression of MyoD expression in rat skeletal muscle progenitor cell culture, which comprises both myogenic satellite cells and mesenchymal progenitor cells, resulted in diminished myotube formation and an unexpected spontaneous appearance of white adipocytes. Suppressing myomaker expression also resulted in complete absence of myotube formation without reducing MyoD expression, but no adipogenesis was seen in this scenario, indicating that decline in MyoD expression rather than decreased myotube formation is necessary to induce adipogenesis. In addition, spontaneous adipogenesis induced by suppressing MyoD expression in culture was inhibited by the conditioned medium from control culture, indicating that anti\u2010adipogenic factor(s) are secreted from MyoD\u2010positive myogenic cells. These results indicate the presence of regulatory mechanism on adipogenesis by myogenic cells.<\/jats:p>","DOI":"10.1111\/asj.13573","type":"journal-article","created":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T05:41:20Z","timestamp":1625636480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Suppression of MyoD induces spontaneous adipogenesis in skeletal muscle progenitor cell culture"],"prefix":"10.1111","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1491-5127","authenticated-orcid":false,"given":"Keitaro","family":"Yamanouchi","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Physiology, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyuki","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Physiology, Graduate 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