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The Cre mouse line with progesterone receptor promoter (\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>Cre<\/jats:italic>\n                    <\/jats:sup>\n                    ) has been widely used to produce specific uterine gene\u2010deficient mice, but in the Cre line, endogenous\n                    <jats:italic>Pgr<\/jats:italic>\n                    gene is replaced by Cre recombinase gene, which makes the breeding of homozygous mice (\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>Cre\/Cre<\/jats:italic>\n                    <\/jats:sup>\n                    ) difficult because they are infertile. Yang et al. (2013,\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/10.1016\/j.cell.2013.04.017\">https:\/\/10.1016\/j.cell.2013.04.017<\/jats:ext-link>\n                    ) reported the generation of another\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>iresCre<\/jats:italic>\n                    <\/jats:sup>\n                    mouse line that still has endogenous\n                    <jats:italic>Pgr<\/jats:italic>\n                    gene, and they inserted Cre recombinase downstream of the\n                    <jats:italic>Pgr<\/jats:italic>\n                    gene via an internal ribosome entry site (IRES). It is possible that this new\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>iresCre<\/jats:italic>\n                    <\/jats:sup>\n                    line would be useful for uterine research as the mice can be bred as homozygotes (\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>iresCre\/iresCre<\/jats:italic>\n                    <\/jats:sup>\n                    ). Herein, we confirmed the\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>iresCre<\/jats:italic>\n                    <\/jats:sup>\n                    mice effectively directed recombination in the female reproductive tract and was capable of genetic alteration in the endometrium that enables the studies of its uterine function. Our findings demonstrate that the new\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>iresCre<\/jats:italic>\n                    <\/jats:sup>\n                    mouse line is also useful for the generation of uterine\u2010specific knockout mice. The findings using\n                    <jats:italic>Pgr<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>iresCre<\/jats:italic>\n                    <\/jats:sup>\n                    mouse will contribute to the understanding of reproductive systems and diseases in humans and domestic animals.\n                  <\/jats:p>","DOI":"10.1111\/asj.13615","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T02:25:05Z","timestamp":1629167105000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Utility of progesterone receptor\u2010ires\u2010Cre to generate conditional knockout mice for uterine study"],"prefix":"10.1111","volume":"92","author":[{"given":"Takafumi","family":"Namiki","sequence":"first","affiliation":[{"name":"Laboratory of Animal Reproduction, School of Veterinary Medicine Azabu University  Sagamihara Japan"},{"name":"Graduate School of Veterinary Science Azabu University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Kamoshita","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, School of Veterinary Medicine Azabu University  Sagamihara Japan"},{"name":"Graduate School of Veterinary Science Azabu University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Kageyama","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, School of Veterinary Medicine Azabu University  Sagamihara Japan"},{"name":"Graduate School of Veterinary Science Azabu University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7643-7976","authenticated-orcid":false,"given":"Jumpei","family":"Terakawa","sequence":"additional","affiliation":[{"name":"Graduate School of Veterinary Science Azabu University  Sagamihara Japan"},{"name":"Laboratory of Toxicology, School of Veterinary Medicine Azabu University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9398-7358","authenticated-orcid":false,"given":"Junya","family":"Ito","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, School of Veterinary Medicine Azabu University  Sagamihara Japan"},{"name":"Graduate School of Veterinary Science Azabu University  Sagamihara Japan"},{"name":"Center for Human and Animal Symbiosis Science Azabu University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Kashiwazaki","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, School of Veterinary Medicine Azabu University  Sagamihara Japan"},{"name":"Graduate School of Veterinary Science Azabu University  Sagamihara 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