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A total of 180 21\u2010day\u2010old male broilers were randomly assigned to three treatment groups with four replicates per group. The thermoneutral group (TN) (23\u2009\u00b1\u20091\u00b0C) received a basal diet, and another two heat\u2010stressed groups (28\u201335\u201328\u00b0C for 12\u2009h daily) were fed the basal diet (HS) or the basal diet supplemented with probiotic at a dose of 1.5\u2009\u00d7\u200910<jats:sup>8<\/jats:sup>\u2009CFU\/kg (HS_Pro) for 21 consecutive days. Compared with the TN group, the abundance of beneficial bacteria was decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in the caecum of heat\u2010stressed broilers. Heat stress downregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the expression of Toll\u2010like receptor (TLR)2 and upregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the expressions of TLR5, TLR15, avian \u03b2\u2010defensin (AvBD)4, AvBD8, and AvBD14 in the ileum as compared with the TN group. Dietary supplementation of probiotic upregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the occludin expression in the ileum, improved the microbiota balance in the caecum, and decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the gene expressions of TLR5 and TLR15 in the ileum of heat\u2010stressed broilers. Collectively, dietary probiotic supplementation could promote intestinal barrier function via improving gut microbiota community and regulating innate immunity\u2010associated gene expressions in heat\u2010stressed broilers.<\/jats:p>","DOI":"10.1111\/asj.13719","type":"journal-article","created":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T13:38:54Z","timestamp":1649165934000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effect of probiotic supplementation on the expression of tight junction proteins, innate immunity\u2010associated genes, and microbiota composition of broilers subjected to cyclic heat stress"],"prefix":"10.1111","volume":"93","author":[{"given":"Qiufen","family":"Li","sequence":"first","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang China"},{"name":"Jiangxi Province Key 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