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The control and enhancement of sperm function are required for the efficient reproduction of pigs. We previously reported that gamma\u2010aminobutyric acid (GABA) enhanced sperm capacitation and acrosome reaction in mice. In this study, we demonstrated the presence of GABA<jats:sub>A<\/jats:sub> receptors in porcine sperm acrosome. Furthermore, we investigated the GABA effects on porcine sperm function. We did not detect any marked effect of GABA on sperm motility and tyrosine phosphorylation of sperm proteins. However, GABA promoted acrosome reaction, which was suppressed by a selective GABA<jats:sub>A<\/jats:sub> receptor antagonist. GABA binds to GABA<jats:sub>A<\/jats:sub> receptors, resulting in chloride ion influx. We found that treatment with 1\u2009\u03bcM GABA increased the intracellular concentration of chloride ion in the sperm. In addition, the GABA concentration effective in the acrosome reaction was correlated with the porcine sperm concentration. These results indicate that GABA and its receptors can act as modulators of acrosome reaction. This study is the first to report the effects of GABA on porcine sperm function.<\/jats:p>","DOI":"10.1111\/asj.13744","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T14:35:15Z","timestamp":1655217315000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Exogenous gamma\u2010aminobutyric acid addition enhances porcine sperm acrosome reaction"],"prefix":"10.1111","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9379-8829","authenticated-orcid":false,"given":"Shouhei","family":"Kurata","sequence":"first","affiliation":[{"name":"Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science Tohoku University  Sendai Miyagi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohei","family":"Umezu","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Baylor College of 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