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We investigated the relative expression, alternative polyadenylation (APA), and changes in poly(A) tail length of <jats:italic>EEF1A1<\/jats:italic> mRNA in the endometrial caruncle (CAR) and intercaruncle (ICAR) at early and mid\u2010gestation in Japanese Black cattle. The relative <jats:italic>EEF1A1<\/jats:italic> mRNA expression levels in the CAR were the highest on Gestation day 20 and were significantly decreased at mid\u2010gestation. The expression levels in the ICAR were significantly higher than those in the CAR, and the gestation stage had no significant impact. Four different <jats:italic>EEF1A1<\/jats:italic> transcripts with distinct 3\u2032 untranslated regions (UTRs) (proximal and distal types) and poly(A) tails (medium and short types) of different lengths were identified. The <jats:italic>EEF1A1<\/jats:italic> mRNAs with distal 3\u2032 UTR and medium\u2010length poly(A) tails were specific from the CAR of uterus horn at early gestation. RNA\u2010sequencing data analyses revealed that the <jats:italic>HSF1<\/jats:italic>, <jats:italic>MZF1<\/jats:italic>, <jats:italic>E47<\/jats:italic>, <jats:italic>SRF<\/jats:italic>, <jats:italic>GATA2<\/jats:italic>, <jats:italic>GATA3<\/jats:italic>, <jats:italic>GATA6<\/jats:italic>, <jats:italic>HNF\u20103 beta<\/jats:italic> (<jats:italic>FOXA2<\/jats:italic>), <jats:italic>CPSF1<\/jats:italic>, and <jats:italic>Ataxin\u20102<\/jats:italic> genes might affect the <jats:italic>EEF1A1<\/jats:italic> gene expression or poly(A) length.<\/jats:p>","DOI":"10.1111\/asj.13746","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T07:20:24Z","timestamp":1657092024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression and differential posttranscriptional regulation of the <i>elongation factor 1 alpha 1<\/i> gene in endometrial caruncle and intercaruncle of Japanese Black cattle at early and mid\u2010gestation stages"],"prefix":"10.1111","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8545-193X","authenticated-orcid":false,"given":"Masanori","family":"Komatsu","sequence":"first","affiliation":[{"name":"Komatsu Laboratory of Computational Biology for Domestic Animals  Ryugasaki Japan"},{"name":"Division of Tear\u2010Round Grazing Research, Western Region Agricultural Research Center NARO  Oda Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5627-7082","authenticated-orcid":false,"given":"Hideyuki","family":"Mannen","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science Kobe University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8194-3577","authenticated-orcid":false,"given":"Masaaki","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Division of Animal Breeding and Reproduction Research, Institute of Livestock and Grassland Science National Agriculture Food Research Organization (NARO)  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0061-207X","authenticated-orcid":false,"given":"Kazunaga","family":"Oshima","sequence":"additional","affiliation":[{"name":"Division of Tear\u2010Round Grazing Research, Western Region Agricultural Research Center NARO  Oda Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2015.00075"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(04)73186-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1520945114"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6271"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(00)74856-9"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.07.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.5.1311"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.224202"},{"key":"e_1_2_7_10_1","first-page":"367","article-title":"Mechanisms of receptivity and implantation in cows: Projection from basic research","volume":"68","author":"Hashizume K.","year":"2015","journal-title":"Nihon Juishikai Zashi"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.013835"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.133900"},{"key":"e_1_2_7_13_1","first-page":"229","volume-title":"Reproduction in Farm Animals","author":"Jainudeen M. 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