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Our aim was to compare the efficacies of two meiotic inhibitors, dibutyryl\u2010cAMP and roscovitine for the meiosis synchronization during in vitro maturation (IVM) of porcine oocytes vitrified at the germinal vesicle (GV) stage. We first compared the efficacy of 1\u2009mM dibutyryl\u2010cAMP and 25\u2009\u03bcM roscovitine on meiotic arrest during the first 22\u2009h of IVM. Dibutyryl\u2010cAMP could maintain the GV stage in 83.5% of oocytes; however, roscovitine was even more effective (96.6%), whereas only 17.4% of the oocytes remained at the GV stage without these additives. Temporal meiotic arrest for 22\u2009h by roscovitine did not reduce the percentage of oocytes reaching the Metaphase II stage during subsequent IVM. However, after parthenogenetic stimulation or in vitro fertilization, subsequent embryo development to the blastocyst stage was compromised after roscovitine treatment, whereas dibutyryl\u2010cAMP improved the percentage of blastocyst development. In conclusion, dibutyryl\u2010cAMP could derogate but not completely prevent premature meiosis in vitrified oocytes, whereas roscovitine could more efficiently prevent it. However, for embryo production, the use of roscovitine was disadvantageous, whereas the use of dibutyryl\u2010cAMP was beneficial.<\/jats:p>","DOI":"10.1111\/asj.13795","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T09:41:21Z","timestamp":1671788481000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Dibutyryl\u2010cAMP and roscovitine differently affect premature meiotic resumption and embryo development of vitrified immature porcine oocytes"],"prefix":"10.1111","volume":"93","author":[{"given":"Hiep Thi","family":"Nguyen","sequence":"first","affiliation":[{"name":"Institute of Agrobiological Sciences National Agriculture and Food Research Organization (NARO)  Tsukuba Ibaraki Japan"},{"name":"Institute of Biotechnology (IBT) Vietnam Academy of Science and Technology (VAST)  Hanoi Vietnam"},{"name":"The United Graduate School of Veterinary Science Yamaguchi University  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