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Porcine spermatozoa were cryopreserved in a freezing extender containing 3% (v\/v) glycerol and various concentrations of DMGA\u2010PLL. At 12\u2009h after thawing, the motility index of spermatozoa cryopreserved with 0.25% (v\/v) DMGA\u2010PLL (25.9) was significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) higher than that of spermatozoa cryopreserved with 0%, 0.125%, or 0.5% DMGA\u2010PLL (10.0\u201316.3). In addition, the blastocyst formation rate of embryos derived from spermatozoa cryopreserved with 0.25% DMGA\u2010PLL (22.8%) was significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) higher than that of embryos derived from spermatozoa cryopreserved with 0%, 0.125%, or 0.5% DMGA\u2010PLL (7.9%\u201310.9%). The mean number of total piglets born to sows inseminated with spermatozoa cryopreserved without DMGA\u2010PLL (9.0) was significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) lower than that of total piglets born to sows inseminated with spermatozoa stored at 17\u00b0C (13.8). However, when spermatozoa cryopreserved with 0.25% DMGA\u2010PLL were used for artificial insemination, the mean number of total piglets (11.7) was not significantly different from that obtained following artificial insemination using spermatozoa stored at 17\u00b0C. The results showed the usefulness of DMGA\u2010PLL as a cryoprotectant in the cryopreservation of porcine spermatozoa.<\/jats:p>","DOI":"10.1111\/asj.13821","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T12:47:13Z","timestamp":1677847633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Improved fertility of frozen\u2010thawed porcine spermatozoa with 3,3\u2010dimethylglutaric anhydride poly\u2010L\u2010lysine as a novel cryoprotectant"],"prefix":"10.1111","volume":"94","author":[{"given":"Hansol","family":"Jin","sequence":"first","affiliation":[{"name":"Laboratory of Animal Reproduction, Faculty of Agriculture Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wooyoon","family":"Choi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, Faculty of Agriculture Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuaki","family":"Matsumura","sequence":"additional","affiliation":[{"name":"School of Materials Science Japan Advanced Institute of Science and Technology  Nomi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suong\u2010hyu","family":"Hyon","sequence":"additional","affiliation":[{"name":"BioVerde Incorporated  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Gen","sequence":"additional","affiliation":[{"name":"BioVerde Incorporated  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Hayashi","sequence":"additional","affiliation":[{"name":"BioVerde Incorporated  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadahiro","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Section of Swine Kagoshima Prefectural Economics Federation of Agricultural Cooperatives  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moe","family":"Ijiri","sequence":"additional","affiliation":[{"name":"Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8837-1882","authenticated-orcid":false,"given":"Kazuchika","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, Faculty of Agriculture Kagoshima University  Kagoshima Japan"},{"name":"The United Graduate School of Agricultural Sciences Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.02.026"},{"key":"e_1_2_7_3_1","first-page":"375","article-title":"Cryoprotectants: The essential antifreezes to protect life in the frozen state","volume":"25","author":"Barry J. 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