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We hypothesized that the brain EPls of whales (<jats:styled-content style=\"fixed-case\"><jats:italic>Balaenoptera edeni<\/jats:italic><\/jats:styled-content>), another <jats:italic>Cetartiodactyla<\/jats:italic> with at least twice the lifespan of bovines, could stimulate FSH secretion by gonadotrophs. To test this hypothesis, bovine gonadotrophs (from approximately 2\u2010year\u2010old Japanese Black heifers) were cultured for 3.5\u00a0days and treated with increasing concentrations of brain EP1s from whales (approximately 22\u2009years old). FSH and LH secretion was stimulated by all tested concentrations of whale EPls (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). To clarify the important differences between bovine and whale EPls, we utilized two\u2010dimensional liquid chromatography\u2013mass spectrometry, which revealed 35 peaks. Among them, we observed significant differences between 12 EPl molecular species. Additionally, we identified differentially expressed genes for enzymes involved in EPl synthesis or degradation in the hypothalamus of young heifers and old cows (approximately 10\u00a0years old) as compared to whales (approximately 28\u2009years old) via deep sequencing of the transcriptome. We conclude that whale brains contain unique EPls that stimulate both FSH and LH secretion by bovine gonadotrophs.<\/jats:p>","DOI":"10.1111\/asj.13839","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:44:25Z","timestamp":1685407465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ethanolamine plasmalogens derived from whale brain stimulate both follicle\u2010stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs"],"prefix":"10.1111","volume":"94","author":[{"given":"Risa","family":"Saito","sequence":"first","affiliation":[{"name":"Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8266-5249","authenticated-orcid":false,"given":"Takeharu","family":"Bando","sequence":"additional","affiliation":[{"name":"Institute of Cetacean Research 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