{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:46:15Z","timestamp":1787960775625,"version":"build-2784847793"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":214,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Boar sperm are less resistant to the dramatic environmental changes that occur during in vitro preservation. Spermidine has various physiological functions including the anti\u2010oxidative effect. The main objective of this study was to clarify whether spermidine could protect boar sperm from the attack of reactive oxygen species under cryopreservation treatment. We set the concentrations of spermidine at 0, 2, 4, 6, and 8\u00a0mmol\/L and evaluated the effects of spermidine on sperm motility, viability, malformation rates, kinetic parameters, membrane integrity, mitochondrial activity, DNA integrity, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> content, malondialdehyde content, total antioxidant capacity, and antioxidant enzyme activity. Finally, the effects of spermidine on the sperm fertility were assessed by artificial insemination. The results showed that spermidine improved various physiological parameters of sperm in a dose\u2010dependent manner. The quality and antioxidant capacity of sperm cryopreserved with 6\u00a0mmol\/L spermidine were significantly less reduced (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), and the contents of malformation rate, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, and malondialdehyde content were significantly decreased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The significant increase in the number of litters indicated the possibility that spermidine had important practical value in pig reproduction (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Therefore, the addition of appropriate concentrations of spermidine to cryopreservation extenders may effectively improve the quality of boar sperm for in vitro preservation.<\/jats:p>","DOI":"10.1111\/asj.13859","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T04:29:32Z","timestamp":1691123372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Exogenous spermidine effectively improves the quality of cryopreserved boar sperm"],"prefix":"10.1111","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5609-1621","authenticated-orcid":false,"given":"Jingchun","family":"Li","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University  Daqing 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