{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:46:07Z","timestamp":1789595167743,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T00:00:00Z","timestamp":1699920000000},"content-version":"vor","delay-in-days":317,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the metabolic effects of propylene glycol (PG) over 60, 90, and 120\u2009days in lambs. Seventy\u2010two weaned male lambs were allocated into three groups: control (Con), PG1.5 (1.5\u2009mL\/kg live weight<jats:sup>0.75<\/jats:sup>), and PG3 (3\u00a0mL\/kg live weight<jats:sup>0.75<\/jats:sup>). Blood samples were collected at the beginning and slaughter days. Biochemical parameters (glucose, triglycerides, ALT, AST, LDH, BUN, and insulin) and gene and protein levels of peroxisome proliferator activated receptor gamma (PPAR\u03b3), diacylglycerol o\u2010acyltransferase 1 (DGAT1), carbohydrate responsive element binding protein (ChREBP), and sterol regulatory element binding transcription factor 1c (SREBP\u20101c) in the liver were determined. Glucose in PG1.5 was increased on Day 60, while significant differences were observed in biochemical parameters except for insulin on the 60, 90, and 120\u2009days. Biochemical parameters such as ALT, AST, LDH, and BUN increased over time, while triglycerides decreased. DGAT1 gene and protein levels were lower, while SREBP\u20101c and PPAR\u03b3 were higher in PG groups on Day 60. While <jats:italic>SREBP\u20101c<\/jats:italic> was lower in PG1.5, ChREBP was higher in PG3 on Day 90. <jats:italic>PPAR\u03b3<\/jats:italic>, <jats:italic>DGAT1<\/jats:italic>, and <jats:italic>ChREBP<\/jats:italic> were upregulated in PG3 on Day 120. Positive correlations were found between proteins. The long\u2010term use of PG in lambs did not have detrimental effects on metabolism. The study provides valuable insights into the molecular mechanisms underlying the metabolic effects of PG in lambs, shedding light on its potential applications in lamb production.<\/jats:p>","DOI":"10.1111\/asj.13886","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:43:05Z","timestamp":1700008985000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of propylene glycol used at different doses in Akkaraman lambs rations on metabolism\u2010related parameters and liver gene and protein expression during different feeding periods"],"prefix":"10.1111","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9248-828X","authenticated-orcid":false,"given":"Ak\u0131n","family":"Yakan","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Genetics Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"\u00d6zkan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Genetics Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ufuk","family":"Kaya","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Biostatistics Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasan H\u00fcseyin","family":"Ke\u00e7eli","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Genetics Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevda","family":"Dalkiran","sequence":"additional","affiliation":[{"name":"Institute of Health Sciences, Department of Molecular Biochemistry and Genetics Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irem","family":"Karaaslan","sequence":"additional","affiliation":[{"name":"Technology and Research &amp; 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