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In our study, we observed two types of nucleolar morphology in mouse in vitro fertilized embryos at the four\u2010cell stage, which we refer to single nucleolus (SN) and multiple nucleoli (MN). To visualize nucleolar morphology, four\u2010cell embryos were immunostained with anti\u2010NOPP140 antibody. These embryos were categorized into five types based on the number of blastomeres carrying SN: SN4\/MN0, SN3\/MN1, SN2\/MN2, SN1\/MN3, and SN0\/MN4, with percentages of 13, 27, 21, 23 and 9, respectively. Next, using a light microscope, we divided the four\u2010cell in vitro fertilized embryos without fixation into two groups: those with at least two blastomeres displaying SN (SN embryos) and those without (MN embryos). Notably, significantly more SN embryos developed into blastocysts and offspring at 18.5 dpc compared with MN embryos. Furthermore, SN embryos displayed a higher NANOG\u2010positive cell number at the blastocyst stage, significantly lower body and placental weights, resulting in a higher fetal\/placental ratio. These findings suggest a close association between nucleolar state at the four\u2010cell stage and subsequent developmental potential.<\/jats:p>","DOI":"10.1111\/asj.13907","type":"journal-article","created":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T09:51:18Z","timestamp":1702720278000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Heterogeneity of nucleolar morphology in four\u2010cell mouse embryos after IVF: association with developmental potential"],"prefix":"10.1111","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0813-3081","authenticated-orcid":false,"given":"Md Wasim","family":"Bari","sequence":"first","affiliation":[{"name":"Department of Integrated Applied Life Science Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences University of Yamanashi  Kofu 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