{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:20:32Z","timestamp":1759774832109,"version":"3.41.2"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":213,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To improve the fertility of cervical artificial insemination (AI) in sheep, we investigated isoxsuprine HCl usage on the cervical passage during cervical AI. We also compared cervical and laparoscopic AI fertility results of using chilled semen at different durations. Semen was collected from rams and diluted as 20\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> or 400\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> spermatozoa\/straw for laparoscopic and cervical AI, respectively, and chilled to 4\u00b0C within 2\u2009h. Sheep were inseminated with chilled semen for 8 or 24 h via the laparoscopic or cervical AI method. Moreover, some of the cervical inseminated sheep were injected intramuscularly with 0.5\u2009mg\/kg of isoxsuprine HCl 15\u2009min before AI. As a result, the use of isoxsuprine HCl did not affect cervical transit and fertility. In addition, fertility was affected by the storage duration of the semen; laparoscopic AI was more successful than cervical AI in terms of fertility; if cervical AI is performed, the duration between semen collection and AI should be less than 8\u2009h after chilling the semen at 4\u00b0C, and if laparoscopic AI is performed, the time between semen collection and insemination can be up to 24 h after chilling the semen at 4\u00b0C. Longer storage periods should be studied.<\/jats:p>","DOI":"10.1111\/asj.13973","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T08:42:46Z","timestamp":1722501766000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Failure of isoxsuprine HCl to increase sheep fertility after cervical versus laparoscopic AI using chilled semen at different durations"],"prefix":"10.1111","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7130-8796","authenticated-orcid":false,"given":"B\u00fclent","family":"B\u00fclb\u00fcl","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Dokuz Eyl\u00fcl University  \u0130zmir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cemal","family":"Dayan\u0131kl\u0131","sequence":"additional","affiliation":[{"name":"Department of Breeding Techniques Sheep Breeding Research Institute  Bal\u0131kesir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebru","family":"\u015eeng\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Breeding Techniques Sheep Breeding Research Institute  Bal\u0131kesir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayd\u0131n","family":"Turan","sequence":"additional","affiliation":[{"name":"Department of Breeding Techniques Sheep Breeding Research Institute  Bal\u0131kesir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammet","family":"Aksoy","sequence":"additional","affiliation":[{"name":"Department of Breeding Techniques Sheep Breeding Research Institute  Bal\u0131kesir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zekariya","family":"Nur","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Bursa Uluda\u011f University  Bursa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13339"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13523"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.001"},{"key":"e_1_2_8_5_1","first-page":"365","volume-title":"Reproduction in Farm Animals","author":"Ax R. 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