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On \u221231\u2009days before the artificial insemination protocol, 562 lactating, multiparous cows were assigned to receive (MG) or not (CG) RPM supplementation (3\u2009g\/cow mixed into 100\u2009g of mineral supplement). Both groups remained in tropical pastures and received supplementation for 77\u2009days. A subset of cows (<jats:italic>n<\/jats:italic>\u00a0=\u2009142) remained with an intravaginal thermometer collecting intravaginal temperature (IT). The respective minimum, average, and maximum environmental THI were 72.8, 78.0, and 83.3. Effects of treatment\u2009\u00d7\u2009hour of the day were detected (<jats:italic>P<\/jats:italic>\u00a0&lt;\u20090.0001) for IT. From 1330 to 1730\u2009h and 1830 to 1900\u2009h, IT was higher (<jats:italic>P<\/jats:italic>\u00a0&lt;\u20090.05) for CG versus MG cows when exposed to moderate and high THI. The supplementation with RPM did not affect conception rate (CG\u2009=\u200964.4% vs. MG\u2009=\u200958.2%; <jats:italic>P<\/jats:italic>\u00a0&gt;\u20090.05). In conclusion, 3\u00a0g of RPM supplementation lowered internal body temperature and possibly altered critical THI threshold in Nelore cows with no impact on reproduction.<\/jats:p>","DOI":"10.1111\/asj.13980","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T01:53:21Z","timestamp":1721958801000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Rumen\u2010protected methionine modulates body temperature and reduces the incidence of heat stress temperatures during the hottest hours of the day of grazing heat\u2010stressed <scp><i>Bos indicus<\/i><\/scp> beef cows"],"prefix":"10.1111","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1133-4589","authenticated-orcid":false,"given":"Vinicius de Souza","family":"Izquierdo","sequence":"first","affiliation":[{"name":"Range Cattle Research and Education Center University of Florida  Ona Florida"},{"name":"Center for 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