{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T14:44:55Z","timestamp":1781189095975,"version":"3.54.1"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T00:00:00Z","timestamp":1750291200000},"content-version":"vor","delay-in-days":169,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study investigated the role and mechanism of \u03b1\u2010glycerol monolaurate (\u03b1\u2010GML), a Food and Drug Administration\u2013approved natural emulsifier, in enhancing tight junction (TJ) assembly and intestinal barrier function. In\u00a0vitro experiments showed that \u03b1\u2010GML significantly increased the gene and protein levels of TJ\u2010related occludin (OCLN) and zonula occluden\u20101 (ZO\u20101), increased ZO\u20101 distribution in the cell membrane, and reduced paracellular permeability in porcine jejunum epithelial cells (IPEC\u2010J2). These findings indicated that \u03b1\u2010GML enhanced intestinal barrier function by promoting TJ protein expression and assembly. Compared with the \u03b1\u2010GML group, TJ protein expression and assembly and AMP\u2010activated protein kinase (AMPK) phosphorylation were significantly downregulated in cotreatment groups receiving \u03b1\u2010GML along with a calcium\u2010sensing receptor antagonist or a phospholipase C inhibitor. Notably, the groups treated with \u03b1\u2010GML and inositol 1,4,5\u2010trisphosphate receptor inhibitor showed a significant decrease in TJ assembly and AMPK phosphorylation, with no significant difference in the TJ protein expression levels. In\u00a0vivo experiments demonstrated that dietary \u03b1\u2010GML significantly upregulated TJ levels and AMPK phosphorylation in the jejunum of partridge chicks. In conclusion, \u03b1\u2010GML may upregulate intestinal epithelial TJ assembly via AMPK activation and promote intestinal barrier development in young animals to maintain intestinal health.<\/jats:p>","DOI":"10.1111\/asj.70067","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T23:24:18Z","timestamp":1750375458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["\u03b1\u2010Glycerol Monolaurate Promotes Tight Junction Assembly and Enhances Epithelial Barrier Function in IPEC\u2010J2 Cells and Partridge Chicks"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3294-1555","authenticated-orcid":false,"given":"Siyu","family":"Dai","sequence":"first","affiliation":[{"name":"Guangdong Province Engineering Research Center for Antibody Drug and Immunoassay, College of Life Science and Technology Jinan University  Guangzhou Guangdong 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