{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:51:40Z","timestamp":1773010300025,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":155,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ensuring optimal blood retinol levels of cattle during fattening is essential for achieving high\u2010quality marbling in beef production. We previously developed a rapid analytical method for measuring blood retinol concentration that eliminates the process of blood cell separation. However, achieving a strong correlation with actual retinol levels proved challenging due to the requirements of vigorous agitation of blood and\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane using a vortex mixer. Consequently, in this study, we developed a modified method that does not require intense agitation by adding phosphate\u2010buffered saline (PBS) to the blood and\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane mixture. The addition of PBS facilitated the dispersion of whole blood in\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane, although the augmented interface between the two enhanced extraction efficiency. Furthermore, we conducted field tests using blood samples collected from 98 fattening cattle from six different farms, each using distinct feeding methods and rearing environments, to reflect the diverse feeding practices in Japanese Black beef production. Their retinol concentrations were analyzed using the developed method and compared with values obtained by HPLC. A correlation coefficient of 0.94\u20130.96 was obtained, indicating that blood retinol levels can be reliably measured even among farms with different feeding methods.\n                  <\/jats:p>","DOI":"10.1111\/asj.70069","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T21:55:19Z","timestamp":1749160519000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced Retinol Extraction From Bovine Whole Blood Using\n                    <i>n<\/i>\n                    \u2010Hexane and Phosphate\u2010Buffered Saline"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8336-0172","authenticated-orcid":false,"given":"Wataru","family":"Iwasaki","sequence":"first","affiliation":[{"name":"Integrated Research Center for Wellbeing National Institute of Advanced Industrial Science and Technology (AIST)  Saga Japan"},{"name":"Sensing Technology Research Institute National Institute of Advanced Industrial Science and Technology (AIST)  Saga Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5075-3209","authenticated-orcid":false,"given":"Nobutomo","family":"Morita","sequence":"additional","affiliation":[{"name":"Integrated Research Center for Wellbeing National Institute of Advanced Industrial Science and Technology (AIST)  Saga Japan"},{"name":"Sensing Technology Research Institute National Institute of Advanced Industrial Science and Technology (AIST)  Saga Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Risa","family":"Yokoishi","sequence":"additional","affiliation":[{"name":"Livestock Research Unit Nagasaki Agriculture and Forestry Technical Development Center  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Inoue","sequence":"additional","affiliation":[{"name":"Livestock Research Unit Nagasaki Agriculture and Forestry Technical Development Center  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takanori","family":"Jogi","sequence":"additional","affiliation":[{"name":"Ushio Inc.  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiyu","family":"Miki","sequence":"additional","affiliation":[{"name":"Ushio Inc.  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.60.101"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.59.873"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.61.961"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20104347(91)80476\u2010S"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","unstructured":"Arnett A. M.Daniel andM. E.Dikeman.2008. \u201cRestricting Vitamin A in Cattle Diets Improves Beef Carcass Marbling and USDA Quality and Yield Grades.\u201d","DOI":"10.4148\/2378-5977.1508"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20100436.1987.tb00195.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2007\u20100172"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006\u2010780"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.2527\/af.2014\u20100033"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.12935\/jvma1951.57.371"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13606"},{"key":"e_1_2_5_13_1","unstructured":"Japan Meat Grading Association.2014. \u201cOverview of Beef Carcass Trading Standards.\u201d Japan Meat Grading Association."},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20100436.1982.tb01279.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(04)73203\u20108"},{"key":"e_1_2_5_16_1","unstructured":"Miyamoto T. Y.Ohta S.Moriwaki Y.Takahashi andT.Ohashi.2003. \u201cDetermination of Bovine Serum Retinol by Direct Fluorometric Assay.\u201dhttp:\/\/www.naro.affrc.go.jp\/project\/results\/laboratory\/niah\/2003\/niah03\u201006.html."},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2016.04.026"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.14982\/rsmp.12.179"},{"key":"e_1_2_5_19_1","volume-title":"Japanese Feeding Standard for Beef Cattle","author":"National Agriculture and Food Research Organization (NARO)","year":"2009"},{"key":"e_1_2_5_20_1","unstructured":"National Agriculture and Food Research Organization (NARO).2018. \u201cDetermination of Fat Soluble Vitamins in Serum by HPLC.\u201d Manual of Clinical Biochemical Tests for Biochemical Pathology in Livestock.http:\/\/www.naro.affrc.go.jp\/laboratory\/niah\/disease\/biochem\/hplc_vave\/sokutei\/index.html."},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2015.11.066"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0309\u20101740(97)00086\u20107"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2011\u20104756"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13329"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1071\/ar9921809"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.71.199"}],"container-title":["Animal Science Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.70069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/asj.70069","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.70069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:10:18Z","timestamp":1773004218000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/asj.70069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/asj.70069"],"URL":"https:\/\/doi.org\/10.1111\/asj.70069","archive":["Portico"],"relation":{},"ISSN":["1344-3941","1740-0929"],"issn-type":[{"value":"1344-3941","type":"print"},{"value":"1740-0929","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2023-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70069"}}