{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:13:51Z","timestamp":1791414831739,"version":"4.3.3"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":222,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The study was aimed at examining the effect of maternal IgY levels on embryonic development, gut microbiota colonization, and resistance to\n                    <jats:italic>Salmonella pullorum<\/jats:italic>\n                    infection in offspring chicks. A total of 60 thirty\u2010six\u2010week\u2010old breeders that were sorted from 969 breeders according to their yolk IgY content were allocated to two groups: a high egg yolk IgY levels group (\n                    <jats:bold>HIgY<\/jats:bold>\n                    ) and a low egg yolk IgY levels group (\n                    <jats:bold>LIgY<\/jats:bold>\n                    ). Fertilized eggs of breeders were collected for hatching. On Day 8, offspring chicks were challenged with\n                    <jats:italic>S. pullorum<\/jats:italic>\n                    . Results showed that compared with the LIgY group, the HIgY group exhibited a considerable increase in embryonic weight, significant enhancement in the richness and diversity of gut microbiota, along with an increased relative abundance of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    in 7\u2010day\u2010old chicks (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The bacterial load in the spleen, liver tissue, and cecal contents, the mRNA expression of nitric oxide synthase 2 (\n                    <jats:bold>\n                      <jats:italic>NOS2<\/jats:italic>\n                    <\/jats:bold>\n                    ) and interleukin\u20101\u03b2 (\n                    <jats:bold>\n                      <jats:italic>IL\u20101\u03b2<\/jats:italic>\n                    <\/jats:bold>\n                    ) in the cecal tonsils and spleen of offspring chicks in the HIgY group markedly reduced on 1\u2010day post\u2010infection (\n                    <jats:bold>dpi<\/jats:bold>\n                    ) compared with the LIgY group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), and mucin\u20102 (\n                    <jats:bold>\n                      <jats:italic>MUC2<\/jats:italic>\n                    <\/jats:bold>\n                    ) and\n                    <jats:italic>Occludin<\/jats:italic>\n                    mRNA expression in the jejunum of offspring chicks in the HIgY group was markedly increased compared with the LIgY group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). In summary, maternal IgY increased the richness and diversity of gut microbiota, enhanced the colonization of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    , improved the immunity and intestinal barrier function, thereby increasing the resistance against\n                    <jats:italic>S. pullorum<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.1111\/asj.70093","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T02:33:58Z","timestamp":1754966038000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Maternal IgY Accelerates\n                    <i>Lactobacillus<\/i>\n                    Colonization, Improves Intestinal Barrier Function and\n                    <i>Salmonella pullorum<\/i>\n                    Resistance of Offspring Chicks"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6025-6575","authenticated-orcid":false,"given":"Ya\u2010nan","family":"Ding","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Hunan Agricultural University  Changsha Hunan China"},{"name":"Hunan Engineering Research Center of Poultry Production Safety  Changsha China"},{"name":"Yuelushan Laboratory Hunan Agricultural University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Hunan Agricultural University  Changsha Hunan China"},{"name":"Hunan Engineering Research Center of Poultry Production Safety  Changsha China"},{"name":"Yuelushan Laboratory Hunan Agricultural University  Changsha Hunan 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