{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:52:39Z","timestamp":1773010359713,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"vor","delay-in-days":238,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21KK0124"],"award-info":[{"award-number":["JP21KK0124"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22H02499"],"award-info":[{"award-number":["JP22H02499"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K19896"],"award-info":[{"award-number":["JP22K19896"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K23805"],"award-info":[{"award-number":["JP23K23805"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    As zinc is important for the fertilization competency of sperms and oocytes, we investigated the effects of zinc chloride (ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    ) supplementation during in\u00a0vitro fertilization (IVF) on porcine oocyte fertilization and development. We evaluated the effects of ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    concentration (0, 1, 10, and 20\u2009\u03bcg\/mL) on the quality of frozen\u2010thawed boar spermatozoa cultured for 5\u2009h and on oocyte fertilization and embryo development after IVF. Spermatozoa from three different boars were additionally tested. ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    supplementation effects along with metal chelators, calcium ethylenediaminetetraacetic acid (Ca\u2010EDTA) and zinc EDTA (Zn\u2010EDTA), were also examined. ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    supplementation did not affect the quality of frozen\u2010thawed spermatozoa after culture for 5\u2009h. Supplementation with 1\u2010\u03bcg\/mL ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    decreased the percentage of polyspermic fertilization compared to that at 0\u2010 and 20\u2010\u03bcg\/mL ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    . Moreover, it increased blastocyst formation rate compared to that in the other supplementation groups. In different boar spermatozoa, ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    supplementation (1\u2009\u03bcg\/mL) decreased polyspermic fertilization but did not improve embryo development. Co\u2010incubation with Ca\u2010EDTA did not reduce polyspermic fertilization, but Zn\u2010EDTA co\u2010incubation reduced polyspermic fertilization similar to that with ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    alone. In conclusion, 1\u2010\u03bcg\/mL ZnCl\n                    <jats:sub>2<\/jats:sub>\n                    supplementation during IVF reduces polyspermic fertilization but may not improve embryo development.\n                  <\/jats:p>","DOI":"10.1111\/asj.70095","type":"journal-article","created":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T07:10:08Z","timestamp":1756278608000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zinc Chloride Supplementation During In\u00a0Vitro Fertilization Reduces Polyspermic Fertilization of Porcine Oocytes"],"prefix":"10.1111","volume":"96","author":[{"given":"Supitcha","family":"Kaewma","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science Chulalongkorn University  Bangkok Thailand"},{"name":"Faculty of Veterinary Science Prince of Songkla University  Hat Yai Thailand"},{"name":"Bio\u2010Innovation Research Center Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0382-959X","authenticated-orcid":false,"given":"Takeshige","family":"Otoi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science Chulalongkorn University  Bangkok Thailand"},{"name":"Bio\u2010Innovation Research Center Tokushima University  Tokushima Japan"},{"name":"Faculty of Veterinary Medicine Universitas Airlangga  Surabaya Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oky\u00a0Setyo","family":"Widodo","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Universitas Airlangga  Surabaya Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7045-3922","authenticated-orcid":false,"given":"Megumi","family":"Nagahara","sequence":"additional","affiliation":[{"name":"Bio\u2010Innovation Research Center Tokushima University  Tokushima 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