{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:25:40Z","timestamp":1769646340805,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Embryo recovery and transfer techniques efficiently assist superior cow selection for beef and milk production. In this study, we evaluated the use of vulvar blood flow volume (BFV) measurements obtained with a portable blood flow meter in predicting the follicle number. We explored the relationship between vulvar BFV and follicular hormone dynamics in cows. Eleven Japanese Black cows underwent superovulation treatment, after which vulvar BFV, serum estradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ) and progesterone levels, and follicle number were measured. All measurements were repeated in the luteal phase (7\u2009days before estrus), 1 and 2\u2009days before estrus, and 2 and 7\u2009days after estrus. The vulvar BFV was significantly elevated 1\u2009day before estrus compared with that during the luteal phase. Furthermore, the vulvar BFV dynamics mirrored the follicle number and serum E\n                    <jats:sub>2<\/jats:sub>\n                    level, both of which were significantly elevated 1\u2009day before estrus compared with those during the luteal phase. In addition, positive correlations were observed between vulvar BFV and follicle number, between serum E\n                    <jats:sub>2<\/jats:sub>\n                    level and follicle number, and between vulvar BFV and E\n                    <jats:sub>2<\/jats:sub>\n                    level. Thus, vulvar BFV measurements could serve as a practical and non\u2010invasive strategy for predicting follicle number and may provide a valuable tool for farmers.\n                  <\/jats:p>","DOI":"10.1111\/asj.70146","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T07:51:36Z","timestamp":1767685896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Portable Device\u2013Based Method for Predicting Follicle Number Using Vulvar Blood Flow Volume Measurement in Cows"],"prefix":"10.1111","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7045-3922","authenticated-orcid":false,"given":"Megumi","family":"Nagahara","sequence":"first","affiliation":[{"name":"Bio\u2010Innovation Research Center Tokushima University  Tokushima 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Agriculture, Forestry and Fisheries Technology Support Center  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohei","family":"Torii","sequence":"additional","affiliation":[{"name":"Center for Design\u2010Oriented AI Education and Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oky\u00a0Setyo","family":"Widodo","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Universitas Airlangga  Surabaya Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mao","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Bio\u2010Innovation Research Center Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichiro","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Bio\u2010Innovation Research Center Tokushima University  Tokushima 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